mouse ( Mus musculus )
eomesodermin

Western result: +

GST GST-EOMES (TBR2) A1041a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: EOMES (TBR2)
CBI Antibody Core Code: A1041a
"Lot" Number: 1 Position: 100 (273-372)
% Identical Homology of Target to Mouse Protein: 100% (NP_034266.1| eomesodermin homolog)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
YTASENDRLTPSPTDSPRSHQIVPGGRYGVQNFFPEPFVN
TLPQARYYNGERTVPQTNGLLSPQQSEEVANPPQRWLVTP
VQQPVTNKLDIGSYESEYTS

Antibody Name/Abbreviation: EOMES (TBR2)
CBI Antibody Core Code: A1041b
"Lot" Number: 1 Position: 20 (470-489)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
CKRKRLSPSTPSNGNSPPIK

Sequence Name: eomesodermin
Full name: eomesodermin homolog
UniGene Number: N.A.
Accession Number: NP_034266; XP_135209
Source organism: mouse ( Mus musculus )
Full length size (aa): 516
NCBI information page: click here

Full length sequence:
MLPPGGFPAAVCPPARAQFGPAAGSGSGAGSSGGGAGGPGAYPYGQGSPL
YGPYAGTSAAGSCGGLGGLGVPGSGFRAHVYLCNRPLWLKFHRHQTEMII
TKQGRRMFPFLSFNINGLNPTAHYNVFVEVVLADPNHWRFQGGKWVTCGK
ADNNMQGNKMYVHPESPNTGSHWMRQEISFGKLKLTNNKGANNNNTQMIV
LQSLHKYQPRLHIVEVTEDGVEDLNEPSKTQTFTFSETQFIAVTAYQNTD
ITQLKIDHNPFAKGFRDNYDSMYTASENDRLTPSPTDSPRSHQIVPGGRY
GVQNFFPEPFVNTLPQARYYNGERTVPQTNGLLSPQQSEEVANPPQRWLV
TPVQQPVTNKLDIGSYESEYTSSTLLPYGIKSLPLQTSHALGYYPDPTFP
AMAGWGGRGAYQRKMAAGLPWTSRMSPPVFPEDQLAKEKVKEEISSSWIE
TPPSIKSLDSSDSGVYNSACKRKRLSPSTPSNGNSPPIKCEDINTEEYSK
DTSKGMGAYYAFYTSP