mouse ( Mus musculus )
mISR

Western result: +

control mISR A1044b (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: mISR
CBI Antibody Core Code: A1044b
"Lot" Number: 1 Position: 20 (560-579)
% Identical Homology of Target to Mouse Protein: 100% (NP_034698.1| insulin receptor)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
DIDPPQRSNDPKSQTPSHPG

Antibody Name/Abbreviation: mISR
CBI Antibody Core Code: A1044a
"Lot" Number: 2 Position: 100 (1273-1372)
% Identical Homology of Target to Mouse Protein: 77% (NP_034698.1| insulin receptor)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
FLEIVNLLKDDLHPSFPEVSFFYSEENKAPESEELEMEFE
DMENVPLDRSSHCQREEAGGREGGSSLSIKRTYDEHIPYT
HMNGGKKNGRVLTLPRSNPS

Other western:
Code: A1044a
Lot: 1

Western result: -

GST GST-mISR (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: mISR
Full name: insulin receptor
UniGene Number: N.A.
Accession Number: NM_010568
Source organism: mouse ( Mus musculus )
Full length size (aa): 1372
NCBI information page:

Full length sequence:
MGFGRGCETTAVPLLVAVAALLVGTAGHLYPGEVCPGMDIRNNLTRLHEL
ENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLK
DLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNN
ELCYLATIDWSRILDSVEDNYIVLNKDDNEECGDVCPGTAKGKTNCPATV
INGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHKECLGNCSEPDDPT
KCVACRNFYLDGQCVETCPPPYYHFQDWRCVNFSFCQDLHFKCRNSRKPG
CHQYVIHNNKCIPECPSGYTMNSSNLMCTPCLGPCPKVCQILEGEKTIDS
VTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEISGFLKIRRS
YALVSLSFFRKLHLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTITQ
GKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELL
KFSFIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDA
CGSNSWTVVDIDPPQRSNDPKSQTPSHPGWLMRGLKPWTQYAIFVKTLVT
FSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDP
NGNITHYLVYWERQAEDSELFELDYCLKGLKLPSRTWSPPFESDDSQKHN
QSEYDDSASECCSCPKTDSQILKELEESSFRKTFEDYLHNVVFVPRPSRK
RRSLEEVGNVTATTLTLPDFPNVSSTIVPTSQEEHRPFEKVVNKESLVIS
GLRHFTGYRIELQACNQDSPDERCSVAAYVSARTMPEAKADDIVGPVTHE
IFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERG
CRLRGLSPGNYSVRVRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIG
PLIFVFLFSVVIGSIYLFLRKRQPDGPMGPLYASSNPEYLSASDVFPSSV
YVPDEWEVPREKITLLRELGQGSFGMVYEGNAKDIIKGEAETRVAVKTVN
ESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPMLVVMELMAHGD
LKSHLRSLRPDAENNPGRPPPTLQEMIQMTAEIADGMAYLNAKKFVHRDL
AARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKD
GVFTASSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDPPDN
CPERLTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFYSEENK
APESEELEMEFEDMENVPLDRSSHCQREEAGGREGGSSLSIKRTYDEHIP
YTHMNGGKKNGRVLTLPRSNPS