mouse ( Mus musculus )
xCT

Western result: +

GST GST-xCT A1151a (fragment)

18.95 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: xCT
CBI Antibody Core Code: A1151a
"Lot" Number: 1 Position: 45 (1-45)
% Identical Homology of Target to Mouse Protein: 100% (NP_036120.1| solute carrier family 7 (cationic amino acid transporter, y+ system), member 11; sodium independent anionic amino acid transport system; System x; amino acid transport system xc-; cystine/glutamate transporter)
Molecular Weight of fusion protein: 18.95 KDa

Target sequence used to make this antibody:
MVRKPVVATISKGGYLQGNMSGRLPSMGDQEPPGQEKVVL
KKKIT

Antibody Name/Abbreviation: xCT-alt
CBI Antibody Core Code: A1152
"Lot" Number: 1 Position: 21 (482-502)
% Identical Homology of Target to Mouse Protein: 100% (NP_036120.1| solute carrier family 7 (cationic amino acid transporter, y+ system), member 11; sodium independent anionic amino acid transport system; System x; amino acid transport system xc-; cystine/glutamate transporter)
Molecular Weight of fusion protein: 32.31 KDa

Target sequence used to make this antibody:
DRITRTLQIILEVVPEDSKEL

Sequence Name: xCT
Full name: SLC7A11
UniGene Number: N.A.
Accession Number: Q9WTR6
Source organism: mouse ( Mus musculus )
Full length size (aa): 502
NCBI information page:

Full length sequence:
MVRKPVVATISKGGYLQGNMSGRLPSMGDQEPPGQEKVVLKKKITLLRGV
SIIIGTVIGSGIFISPKGILQNTGSVGMSLVFWSACGVLSLFGALSYAEL
GTSIKKSGGHYTYILEVFGPLLAFVRVWVELLVIRPGATAVISLAFGRYI
LEPFFIQCEIPELAIKLVTAVGITVVMVLNSTSVSWSARIQIFLTFCKLT
AILIIIVPGVIQLIKGQTHHFKDAFSGRDTSLMGLPLAFYYGMYAYAGWF
YLNFITEEVDNPEKTIPLAICISMAIITVGYVLTNVAYFTTISAEELLQS
SAVAVTFSERLLGKFSLAVPIFVALSCFGSMNGGVFAVSRLFYVASREGH
LPEILSMIHVHKHTPLPAVIVLHPLTMVMLFSGDLYSLLNFLSFARWLFM
GLAVAGLIYLRYKRPDMHRPFKVPLFIPALFSFTCLFMVVLSLYSDPFST
GVGFLITLTGVPAYYLFIVWDKKPKWFRRLSDRITRTLQIILEVVPEDSK
EL