mouse ( Mus musculus )
HMG box transcription factor 21

Western result: +

GST GST-Sox21 (fragment)

20.49 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Sox21
CBI Antibody Core Code: A1192
"Lot" Number: 1 Position: 59 (219-277)
% Identical Homology of Target to Mouse Protein: 100% (NP_808421.1| SRY-box 21; HMG-box protein SOX21; DNA-binding protein SOX21)
Molecular Weight of fusion protein: 20.49 KDa

Target sequence used to make this antibody:
AAGGHTHSHPSPGNPGYMIPCNCSAWPSPGLQPPLAYILL
PGMGKPQLDPYPAAYAAAL

Sequence Name: HMG box transcription factor 21
Full name:
UniGene Number: N.A.
Accession Number: BC069176
Source organism: mouse ( Mus musculus )
Full length size (aa): 277
NCBI information page:

Full length sequence:
mskpvdhvkrpmnafmvwsraqrrkmaqenpkmhnseiskrlgaewkllt
esekrpfideakrlramhmkehpdykyrprrkpktllkkdkfafpvpygl
gsvadaehpalkagaglhagaggglvpesllanpekaaaaaaaaaarvff
pqpaaaaaaaaaaaaaagspyslldlgskmaeisssssglpyasslgypt
agagafhgaaaaaaaaaaaagghthshpspgnpgymipcncsawpspglq
pplayillpgmgkpqldpypaayaaal