mouse ( Mus musculus )
HMG box transcription factor 1

Western result: +

GST GST-Sox1 (fragment)

21.15 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Sox1
CBI Antibody Core Code: A1194
"Lot" Number: 1 Position: 65 (210-274)
% Identical Homology of Target to Mouse Protein: 100% (NP_033259.1| SRY (sex determining region Y)-box 1)
Molecular Weight of fusion protein: 21.15 KDa

Target sequence used to make this antibody:
QLAYGQHPGAGGRHPHAHPAHPHPHHPHAHPHNPQPMHRY
DMGALQYSPISNSQGYMSASPSGYG

Sequence Name: HMG box transcription factor 1
Full name:
UniGene Number: N.A.
Accession Number: NM_009233
Source organism: mouse ( Mus musculus )
Full length size (aa): 391
NCBI information page:

Full length sequence:
mysmmmetdlhspggaqaptnlsgpagarggggggggggggggtkanqdr
vkrpmnafmvwsrgqrrkmaqenpkmhnseiskrlgaewkvmseaekrpf
ideakrlralhmkehpdykyrprrktktllkkdkyslaggllaagagggg
aavamgvgvgvgaaavgqrlespggaagggyahvngwangaypgsvaaaa
aaaammqeaqlaygqhpgaggrhphahpahphphhphahphnpqpmhryd
mgalqyspisnsqgymsaspsgyggipygaaaaaaaaaggahqnsavaaa
aaaaaassgalgalgslvksepsgsppapahsrapcpgdlremismylpa
geggdpaaaaaaaaqsrlhslpqhyqgagagvngtvplthi