mouse ( Mus musculus )
calcyon

Western result: +

GST GST-calcyon (fragment)

22.8 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: calcyon
CBI Antibody Core Code: A1253
"Lot" Number: 1 Position: 80 (2-81)
% Identical Homology of Target to Mouse Protein: 100% (NP_081045.2| calcyon protein; D1 dopamine receptor-interacting protein; calcyon; D1 dopamine receptor-interacting protein)
Molecular Weight of fusion protein: 22.8 KDa

Target sequence used to make this antibody:
vklgcsfsgkpgkeagdqdgaamdsvplispldvsqlqps
fsdqvvintqteyqltsadqpkkfadlegqrlacshseeg

Sequence Name: calcyon
Full name: D1 dopamine receptor-interacting protein calcyon
UniGene Number: N.A.
Accession Number: Q9DCA7
Source organism: mouse ( Mus musculus )
Full length size (aa): 226
NCBI information page: click here

Full length sequence:
mvklgcsfsgkpgkeagdqdgaamdsvplispldvsqlqpsfsdqvvint
qteyqltsadqpkkfadlegqrlacshseegrrlptarmiafamallgcv
limykaiwydqftcpdgfllrhkictpltlemyytemdperhrsilaaig
ayplsrkhgtempavwgnnyrtakeehkgttpaamavstaaaaaaaegte
psgksldtrekedpqkaegvpsqppk