mouse ( Mus musculus )
m14-3-3 zeta

Antibody Name/Abbreviation: m14-3-3 zeta
CBI Antibody Core Code: A0270
"Lot" Number: 2 Position: 100(51-150)
% Identical Homology of Target to Mouse Protein: 100% (NP_035870.1| tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
VVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETEL
RDICNDVLSLLEKFLIPNASQPESKVFYLKMKGDYYRYLA
EVAAGDDKKGIVDQSQQAYQ

Other western:
Code: A0270
Lot: 1

Western result: ++

GST GST-m14-3-3 zeta (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: m14-3-3 zeta
Full name: Tyr/Tryp 5-monoxegenase activation, zeta polypeptide
UniGene Number: N.A.
Accession Number: JC5384
Source organism: mouse ( Mus musculus )
Full length size (aa): 245
NCBI information page: click here

Full length sequence:
MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKN
VVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSL
LEKFLIPNASQPESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQ
EAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAI
AELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDDAEAGEGGEN