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mouse ( Mus musculus )
2610033C09Rik



Western result: +
GST
GST-2610033C09Rik
(fragment)

N.A. kDa (recombinant)
The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: 2610033C09Rik
CBI Antibody Core Code: A0528

"Lot" Number: 2 Position: 100(1-100)
% Identical Homology of Target to Mouse Protein: 67% (NP_080683.2| RIKEN cDNA 2610033C09)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
MPSRRRGPSRQQLSRSALPSIQTLVGGGCGNGTGLRNRNG
NAIGLPVPPTTALITPGPVRHCQIPDLPVDGSLFFEFLFF
IYLLIVLFIQYINIYKTVWW


Other western:
Code: A0528
Lot: 1


Western result: -
GST
GST-2610033C09Rik
(fragment)

N.A. kDa (recombinant)
The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.




Sequence Name: 2610033C09Rik
Full name: RIKEN cDNA 2610033C09
UniGene Number: N.A.
Accession Number: NM_026407
Source organism: mouse ( Mus musculus )
Full length size (aa): 486
NCBI information page: click here

Full length sequence:
MPSRRRGPSRQQLSRSALPSIQTLVGGGCGNGTGLRNRNGNAIGLPVPPT
TALITPGPVRHCQIPDLPVDGSLFFEFLFFIYLLIVLFIQYINIYKTVWW
YPYNHPASCTSLNFHLIDYYLAAFITVMLARRLVWALISEATKAGAASTV
HYTALILARLVLLTLCGWVLCWTLVNLFRSHSVLNLLFLGYPFGVYVPLY
CFHQDSRAHLLLTDYVVQHQAVEEAASNVGSLARSKDFLSLLLESLKEQF
NNATPIPTHSCPLSPDLIRNEVECLKADFNHRIKEVLFNSLFSAYYVAFL
PLCFVKSTQYYDMRWSCEHLIMVWINAFVMLTTQLLPSKYCDLLHKSAAH
LGKWQKLEHGFYSNAPQHIWSENTIWPQGVLVRHSRCLYRAMGPYNVAVP
SDVSHARFYFLFHRPLRVLNLLILIEGSVVFYQLYSLLRSEKWNHTLSMA
LILFCNYYVLFKLLRDRIVLGRAYSYPLNSYELKAN





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© Monday, February 16, 2009 by the CBI Antibody Core and the Center for Biomedical Inventions, University of Texas Southwestern Medical School.