| Western result: + | | GST
 | GST-2610033C09Rik (fragment)
 | | | | | | The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST. | | Antibody Name/Abbreviation: 2610033C09Rik CBI Antibody Core Code: A0528 "Lot" Number: 2 Position: 100(1-100) % Identical Homology of Target to Mouse Protein: 67% (NP_080683.2| RIKEN cDNA 2610033C09) Molecular Weight of fusion protein: N.A. KDa Target sequence used to make this antibody: MPSRRRGPSRQQLSRSALPSIQTLVGGGCGNGTGLRNRNG
NAIGLPVPPTTALITPGPVRHCQIPDLPVDGSLFFEFLFF
IYLLIVLFIQYINIYKTVWW
Other western: Code: A0528 Lot: 1 Western result: - | | GST
 | GST-2610033C09Rik (fragment)
 | | | | | | The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST. | |
| | Sequence Name: 2610033C09Rik Full name: RIKEN cDNA 2610033C09 UniGene Number: N.A. Accession Number: NM_026407 Source organism: mouse ( Mus musculus ) Full length size (aa): 486 NCBI information page: click here Full length sequence: MPSRRRGPSRQQLSRSALPSIQTLVGGGCGNGTGLRNRNGNAIGLPVPPT
TALITPGPVRHCQIPDLPVDGSLFFEFLFFIYLLIVLFIQYINIYKTVWW
YPYNHPASCTSLNFHLIDYYLAAFITVMLARRLVWALISEATKAGAASTV
HYTALILARLVLLTLCGWVLCWTLVNLFRSHSVLNLLFLGYPFGVYVPLY
CFHQDSRAHLLLTDYVVQHQAVEEAASNVGSLARSKDFLSLLLESLKEQF
NNATPIPTHSCPLSPDLIRNEVECLKADFNHRIKEVLFNSLFSAYYVAFL
PLCFVKSTQYYDMRWSCEHLIMVWINAFVMLTTQLLPSKYCDLLHKSAAH
LGKWQKLEHGFYSNAPQHIWSENTIWPQGVLVRHSRCLYRAMGPYNVAVP
SDVSHARFYFLFHRPLRVLNLLILIEGSVVFYQLYSLLRSEKWNHTLSMA
LILFCNYYVLFKLLRDRIVLGRAYSYPLNSYELKAN
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