| Western result: + | | | | | | | | The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST. | | Antibody Name/Abbreviation: 2b4 CBI Antibody Core Code: A0040 "Lot" Number: 1 Position: 33-132 % Identical Homology of Target to Mouse Protein: 100% (NP_061199.1| CD244 natural killer cell receptor 2B4; NK cell receptor 2B4; non MHC restricted killing associated) Molecular Weight of fusion protein: N.A. KDa Target sequence used to make this antibody: PDSSEEVVGVSGKPVQLRPSNIQTKDVSVQWKKTEQGSHR
KIEILNWYNDGPSWSNVSFSDIYGFDYGDFALSIKSAKLQ
DSGHYLLEITNTGGKVCNKN
| | Sequence Name: 2b4 Full name: UniGene Number: N.A. Accession Number: L19057 Source organism: mouse ( Mus musculus ) Full length size (aa): To be posted NCBI information page: click here Full length sequence: |