mouse ( Mus musculus )
a2MG

Western result: ++

GST GST-a2MG (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: a2MG
CBI Antibody Core Code: A0416
"Lot" Number: 1 Position: 100(615-714)
% Identical Homology of Target to Mouse Protein: 100% (NP_783327.1| alpha-2-macroglobulin)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
KAITYLNTGYQRQLNYKHRDGSYSTFGDKPGRSHANTWLT
AFVLKSFAQARRYIFIDESHITQALTWLSQQQKDNGCFRS
SGSLLNNAMKGGVEDEVTLS

Sequence Name: a2MG
Full name: alpha-2-macroglobulin
UniGene Number: N.A.
Accession Number: XP_132782
Source organism: mouse ( Mus musculus )
Full length size (aa): 1086
NCBI information page: click here

Full length sequence:
MIFIGADEANQNINTTTDKNGLARFSINTDDIMGTSLTVRAKYKDSNVCY
GFRWLTEENVEAWHTANAVFSPSRSFVHLESLPYKLRCEQTLAVQAHYIL
NDEAVLERKELVFYYLMMAKGGIVRAGTHVLPVTQGHKKGHFSILISMET
DLAPVARLVLYTILPNGEVVGDTVKYEIEKCLANKVDLVFHPNIGLPATR
AFLSVMASPQSLCGLRAVDQSVLLTKPEAELSASLVYDLLPVKDLTGFPK
GVNQQEEDTNGCLKQNDTYINGILYSPVQNTNEEDMYGFLKDMGLKVFTN
LNIRKPKVCERLGVNKIPAAYHLVSQGHMDAFLESSESPTETTRSYFPET
WIWDLVIVDSTGVAEMEVTVPDTITEWKAGAFCLSNDTGLGLSPVIDFQA
FQPFFVDLTMPYSVIRGEAFTLKATVLNYLQTCIRVGVQLEASPDFLATP
EEKEQKSHCICMNERHTMSWAVIPKSLGNVNFTVSAEALDSKELCRNEVP
VVPERGKKDTIIKSLLVEPEGLENEVTFNSLLCPTGAEVSEQISLKLPSD
VVEESARASVTVLGDILGSAMQNTQDLLKMPYGCGEQNMVLFAPNIYVLD
YLNETEQLTQEIKTKAITYLNTGYQRQLNYKHRDGSYSTFGDKPGRSHAN
TWLTAFVLKSFAQARRYIFIDESHITQALTWLSQQQKDNGCFRSSGSLLN
NAMKGGVEDEVTLSAYITIALLEMSLPVTHPVVRNALFCLDTAWKSARRG
ASGNHVYTKALLAYAFALAGNQDTKKEILKSLDEEAVKEDNSVHWTRAQK
PRVPADLWYQPQAPSAEVEMTAYVLLAYLTTELVPTREDLTAAMLIVKWL
TKQQNSHGGFSSTQDTVVALHALSKYGAATFTRAKKAAHVTIQSSGAFYT
KFQVNNDNQLLLQRVTLPTVPGDYTAKVAGEGCVYLQTSLKYSVLPREKE
FPFALVVQTLPGTCEDLKAHTTFQISLNISYIGSRSDSNMAIADVKMVSG
FIPLKPTVKMLERSVHVSRTEVSNNHVLIYLDKVSNQMLTLFFMVQQDIP
VRDLKPAIVKVYDYYEKDEFAVAKYSAPCSAGYGNA