mouse ( Mus musculus )
alpha1 antitrypsin (AAT)

Western result: +

GST GST-alpha1 antitrypsin (AAT) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: alpha1 antitrypsin (AAT)
CBI Antibody Core Code: A0086
"Lot" Number: 2 Position: To be posted
% Identical Homology of Target to Mouse Protein: 66% (NP_035461.1| chemokine (C-C motif) ligand 12; small inducible cytokine A12)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEA
VIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

Other western:
Code: A0086
Lot: 1

Western result: ++

GST GST-alpha1 antitrypsin (AAT) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: alpha1 antitrypsin (AAT)
Full name: alpha1 antitrypsin (AAT)
UniGene Number: N.A.
Accession Number: P07758
Source organism: mouse ( Mus musculus )
Full length size (aa): 389
NCBI information page: click here

Full length sequence:
mtpsiswgllllaglcclvpsflaedvqetdtsqkdqspasheiatnlgd
faislyrelvhqsntsniffspvsiatafamlslgskgdthtqileglqf
nltqtseadihksfqhllqtlnrpdselqlstgnglfvnndlklvekfle
eaknhyqaevfsvnfaeseeakkvindfvekgtqgkiaeavkkldqdtvf
alanyilfkgkwkkpfdpenteeaefhvdesttvkvpmmtlsgmlhvhhc
stlsswvllmdyagnatavfllpddgkmqhleqtlskeliskfllnrrrr
laqihfprlsisgeynlktlmsplgitrifnngadlsgiteenaplklsq
avhkavltidetgteaaavtvlqmvpmsmppilrfdhpflfiifeehtqs
piflgkvvdpthk