mouse ( Mus musculus )
mCoro1A-II

Western result: +

GST GST-mCoro1A-I (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: mCoro1A-I
CBI Antibody Core Code: A0257
"Lot" Number: 1 Position: 100(171-270)
% Identical Homology of Target to Mouse Protein: 100% (NP_034028.1| coronin, actin binding protein 1A; coronin-like actin binding protein; coronin 1a; coronin 1)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
GPDVHPDTIYSVDWSRDGALICTSCRDKRVRVIEPRKGTV
VAEKDRPHEGTRPVHAVFVSEGKILTTGFSRMSERQVALW
DTKHLEEPLSLQELDTSSGV

Sequence Name: mCoro1A-II
Full name: coronin, actin binding protein
UniGene Number: N.A.
Accession Number: NM_009898
Source organism: mouse ( Mus musculus )
Full length size (aa): 461
NCBI information page: click here

Sequence Notes: mouse Coro1A-I

Full length sequence:
MSRQVVRSSKFRHVFGQPAKADQCYEDVRVSQTTWDSGFCAVNPKFMALI
CEASGGGAFLVLPLGKTGRVDKNVPLVCGHTAPVLDIAWCPHNDNVIASG
SEDCTVMVWEIPDGGLVLPLREPVITLEGHTKRVGIVAWHPTAQNVLLSA
GCDNVILVWDVGTGAAVLTLGPDVHPDTIYSVDWSRDGALICTSCRDKRV
RVIEPRKGTVVAEKDRPHEGTRPVHAVFVSEGKILTTGFSRMSERQVALW
DTKHLEEPLSLQELDTSSGVLLPFFDPDTNIVYLCGKGDSSIRYFEITSE
APFLHYLSMFSSKESQRGMGYMPKRGLEVNKCEIARFYKLHERKCEPIAM
TVPRKSDLFQEDLYPPTAGPDPALTAEEWLGGRDAGPLLISLKDGYVPPK
SRELRVNRGLDSARRRATPEPSGTPSSDTVSRLEEDVRNLNAIVQKLQER
LDRLEETVQAK