mouse ( Mus musculus )
mCtsD-C

Western result: +

GST GST-mCtsD-C (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: mCtsD-C
CBI Antibody Core Code: A0268
"Lot" Number: 1 Position: 100(311-410)
% Identical Homology of Target to Mouse Protein: 100% (NP_034113.1| cathepsin D)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
KAIGAVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPD
KYILKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGS
YYTVFDRDNNRVGFANAVVL

Sequence Name: mCtsD-C
Full name: cathepsin D
UniGene Number: N.A.
Accession Number: NP_034113
Source organism: mouse ( Mus musculus )
Full length size (aa): 410
NCBI information page: click here

Sequence Notes: mouse CtsD

Full length sequence:
MKTPGVLLLILGLLASSSFAIIRIPLRKFTSIRRTMTEVGGSVEDLILKG
PITKYSMQSSPKTTEPVSELLKNYLDAQYYGDIGIGTPPQCFTVVFDTGS
SNLWVPSIHCKILDIACWVHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGY
LSQDTVSVPCKSDQSKARGIKVEKQIFGEATKQPGIVFVAAKFDGILGMG
YPHISVNNVLPVFDNLMQQKLVDKNIFSFYLNRDPEGQPGGELMLGGTDS
KYYHGELSYLNVTRKAYWQVHMDQLEVGNELTLCKGGCEAIVDTGTSLLV
GPVEEVKELQKAIGAVPLIQGEYMIPCEKVSSLPTVYLKLGGKNYELHPD
KYILKVSQGGKTICLSGFMGMDIPPPSGPLWILGDVFIGSYYTVFDRDNN
RVGFANAVVL