mouse ( Mus musculus )
DOT1

Antibody Name/Abbreviation: DOT1
CBI Antibody Core Code: A0235
"Lot" Number: 3 Position: 100(990-1089)
% Identical Homology of Target to Mouse Protein: 100% (NP_955354.1| histone H3 methyltransferase DOT1; histone methyltransferase DOT1L)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
PPLLSQSRNSGPASPAHQLTASPRLSVTTQGSLPDTSKGE
LPSDPAFSDPESEAKRRIVFSISVGASSKQSPSTRHSPLT
SGTRGDCVQSHGQDSRKRSR

Other western:
Code: A0235
Lot: 2

Western result: +/-

GST GST-DOT1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: DOT1
Full name:
UniGene Number: N.A.
Accession Number: XP_125730
Source organism: mouse ( Mus musculus )
Full length size (aa): 1540
NCBI information page: click here

Full length sequence:
MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPD
LKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNT
RPSNGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDE
IKMTEDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDR
EFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVD
HQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLK
GSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRENK
SNATTPTKVPESKAAATEAPADSGAEEEKSGVATVKKPSPSKARKKKLNK
KGRKMAGRKRGRPKKMSAASAERKSKKSQSTLDLLHSPPPAPPSASPQDA
YRAPHSPFYQLPPSTQLHSPNPLLVAPTPPALQKLLESFRIQYLQFLAYT
KTPQYKANLQQLLDQEKEKNTQLLGTAQQLFGHCQAQKEEIRRLFQQKLD
ELGVKALTYNDLIQAQKEISAHNQQLREQSEQLEKDNSELRSQSLRLLRA
RCEELRLDWSTLSLENLRKEKQALRSQISEKQRHCLELQISIVELEKTQR
QQELLQLKSCVPPDDALSLHLRGKGALGRELEADAGRLRLELDCAKISLP
HLSSMSPELSMNGHAASYELCNAASRPSSKQNTPQYLASPLDQEVVPCTP
SHSGRPRLEKLSGLALPDYTRLSPAKIVLRRHLSQDHTGASKAATSEPHP
RPEHPKESSLPYQSPGLSNSMKLSPQDPPLASPATSPLTSEKGSEKGVKE
RAYSSHGETITSLPVSIPLSTVQPNKLPVSIPLASVVLPSRAERARSTPS
PVPQPRDSSATLEKQTGASAHGAGGAGAGSRSLAVAPTGFYAGSVAISGA
LASSPAPLASGMESAVFDESSGPSSLFATMGSRSTPPQHPPLLSQSRNSG
PASPAHQLTASPRLSVTTQGSLPDTSKGELPSDPAFSDPESEAKRRIVFS
ISVGASSKQSPSTRHSPLTSGTRGDCVQSHGQDSRKRSRRKRASAGTPSL
STGVSPKRRALPTVAGLFTQSSGSPLNLNSMVSNINQPLEITAISSPESS
LKSSPTPYQDHDQPPVLRKERPLGLTNGAHYSPLTSDEEPGSEDEPSSAR
IERKIATISLESKSPPKTLENGGGLVGRKSAPSSEPINSSKWKSTFSPIS
DLGLAKAVDSPLQAGSALSHSPLFSFRPSLEEPAAEAKLPTHPRKSFAGS
LGAAEGPSPGTNPPNGLAFSGGLAADLGLHSFNDGASLSHKGPDVTGLSA
SLSFPSQRGKDSTTEANPFLSRRQPEGLGGLKGEGNANKESGESLPLCGP
SDKASLPHGSRASKGRDRELDFKGGHNLFISAAAVPPGGLLGGPGLVTVA
SSAGSATPTAQAPRPFLSTFAPGPQFTLGPMSLQANLGSVAGSSVLQSLF
STVPAAAGLVHVSSTATRLTNSHTMGSFSSGVAGGTVGGN