mouse ( Mus musculus )
HDAC9-C

Western result: +

GST GST-HDAC9 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: HDAC9
CBI Antibody Core Code: A0612
"Lot" Number: 1 Position: 100(41-140)
% Identical Homology of Target to Mouse Protein: 49% (XP_283056.3| similar to histone deacetylase 9 isoform 5; histone deacetylase 7B; histone deacetylase 7; MEF-2 interacting transcription repressor (MITR) protein; histone deacetylase 4/5-related protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
VREKQLQQELLLIQQQQQIQKQLLIAEFQKQHENLTRQHQ
AQLQEHIKELLAIKQQQELLEKEQKLEQQRQEQEVERHRR
EQQLPPLRGKDRGRERAVAS

Sequence Name: HDAC9-C
Full name: Mouse HDAC9
UniGene Number: N.A.
Accession Number: NM_024124
Source organism: mouse ( Mus musculus )
Full length size (aa): 586
NCBI information page: click here

Full length sequence:
MHSMISSVDVKSEVPMGLEPISPLDLRTDLRMMMPVVDPVVREKQLQQEL
LLIQQQQQIQKQLLIAEFQKQHENLTRQHQAQLQEHIKELLAIKQQQELL
EKEQKLEQQRQEQEVERHRREQQLPPLRGKDRGRERAVASTEVKQKLQEF
LLSKSATKDTPTNGKNHSVGRHPKLWAAHHTSLDQSSPPLSGTSPSYKYT
LPGAQDSKDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGNLVT
SFKKRVFEVAESSVSSSSPGSGPSSPNNGPAGNVTENEASALPPTPHPEQ
LVPQQRILIHEDSMNLLSLYTSPSLPNITLGLPAVPSPLNASNSLKDKQK
CETQMLRQGVPLPSQYGSSIAASSSHVHVAMEGKPNSSHQALLQHLLLKE
QMRQQKLLVAGGVPLHPQSPLATKERISPGIRGTHKLPRHRPLNRTQSAP
LPQSTLAQLVIQQQHQQFLEKQKQYQQQIHMNKLLSKSIEQLKQPGSHLE
EAEEELQGDQSMEDRAASKDTSARSDSSACVEDTLGQVGAVKVKEEPVDS
DEDAQIQEMECGEQAAFMQQVIGKDLAPGFVIKVII