mouse ( Mus musculus )
KIAA0219

Antibody Name/Abbreviation: KIAA0219
CBI Antibody Core Code: A0766
"Lot" Number: 2 Position: 100(1610-1709)
% Identical Homology of Target to Mouse Protein: 98% (XP_487831.1| PREDICTED: hypothetical protein XP_487831)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
KYHIETGGGQLPPRLSSLLIKCLQNPCSDIRLVAEKMIWW
ANKEPRHPLEPQTIKPILKALLDNTKDKNTVVRAYSDQAI
VNLLKMRRGEELLQSLSKIL

Other western:
Code: A0766
Lot: 1

Western result: +

GST GST-KIAA0219 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: KIAA0219
Full name: gi|26006117|dbj|BAC41401.1| mKIAA0219 protein [Mus musculus]
UniGene Number: N.A.
Accession Number: AB093217. GI: 26006116
Source organism: mouse ( Mus musculus )
Full length size (aa): 1744
NCBI information page: click here

Full length sequence:
LKPECALDKSWCQEELPVAVRRAVSLLHTHTIPSRVGKGEPDAAPLSAPA
FSLVFPMLKMVLTEMPYHSEEEEEQMAQILQILTVHAQLRASPDTPPERV
DENGPELLPRVAMLRLLTWVIGTGSPRLQVLASDTLTALCASSSGEDGCA
FAEQEEVDVLLAALQSPCASVRETALRGLMELRLVLPSPDTDEKSGLSLL
RRLWVIKFDKEDEIRKLAERLWSTMGLDLQSDLCSLLIDDVIYHEAAVRQ
AGAEALSQAVARYQRQAAEVMGRLMEIYQEKLYRPPPVLDALGRVISESP
PDQWEARCGLALALNKLSQYLDSSQVKPLFQFFVPDALNDRNPDVRKCML
DAALATLNAHGKENVNSLLPVFEEFLKDAPNDASYDAVRQSVVVLMGSLA
KHLDKSDPKVKPIVAKLIAALSTPSQQVQESVASCLPPLVPAVKEDAGGM
IQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQ
DKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVRE
AADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYC
APKQLSSCLPNIVPKLTEVLTDSHVKVQKAGQQALRQIGSVIRNPEILAI
APVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDR
STDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVS
AKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGL
GVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGP
IIPCILKALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDD
LWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIITAL
GVDRRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLF
GLLLGFLASTCADKRTIAARTLGDLVRKLGEKILPEIIPILEEGLRSQKS
DERQGVCIGLSEIMKSTSRDAVLFFSESLVPTARKALCDPLEEVREAAAK
TFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAVKSRVVL
PYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTP
DEQLEMANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNM
YCSRSKADYSSHLRSLVSGLIRLFNDSSPVVLEESWDALNAITKKLDAGN
QLALIEELHKEIRFIGNECKGEHVPGFCLPKRGVTSILPVLREGVLTGSP
EQKEEAAKGLGLVIRLTSADALRPSVVSITGPLIRILGDRFNWTVKAALL
ETLSLLLGKVGIALKPFLPQLQTTFTKALQDSNRGVRLKAADALGKLISI
HVKVDPLFTELLNGIRAVEDPGIRDTMLQALRFVIQGAGSKVDAAIRKNL
VSLLLSMLGHDEDNTRISTAGCLGELCAFLTDEELNTVLQQCLLADVSGI
DWMVRHGRSLALSVAVNVAPSRLCAGRYSNEVQDMILSNAVADRIPIAMS
GIRGMGFLMKYHIETGGGQLPPRLSSLLIKCLQNPCSDIRLVAEKMIWWA
NKEPRHPLEPQTIKPILKALLDNTKDKNTVVRAYSDQAIVNLLKMRRGEE
LLQSLSKILDVASLEALNECSRRSLRKLACQADSVEQVDDTILT