mouse ( Mus musculus )
membrane associated adenylate kinase (AK1)

Western result: ++

GST GST-AK2 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: AK2
CBI Antibody Core Code: 1Ab088
"Lot" Number: 1 Position: 99 (112-210)
% Identical Homology of Target to Mouse Protein: 83% (NP_058591.2| adenylate kinase 2)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
DELMEKRKEELDSVIEFSIQDSLLIRRITGRLIRPKSGRF
YHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKTRLEAYY
TQTTPLVEYYRKRGIHCAI

Sequence Name: membrane associated adenylate kinase (AK1)
Full name: adenylate kinase isozyme 2
UniGene Number: N.A.
Accession Number: BAA77359
Source organism: mouse ( Mus musculus )
Full length size (aa): 232
NCBI information page: click here

Full length sequence:
MAPNVLASEPEIPKGIRAVLLGPPGAGKGTQAPKLAENFCVCHLATGDML
RAMVASGSELRKKLKATMDAGKLVSDEMVVELIEKNLETPSCKNGFLLDG
FPRTVRQAEMLDELMEKRKEELDSVIEFSIQDSLLIRRITGRLIRPKSGR
FYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKTRLEAYYTQTTPLVEY
YRKRGIHCAIDASQTPDIVFASILAAFSKATS