mouse ( Mus musculus )
Neu3

Antibody Name/Abbreviation: Neu3
CBI Antibody Core Code: A0608
"Lot" Number: 2 Position: 100(659-758)
% Identical Homology of Target to Mouse Protein: 100% (NP_766132.1| neurexin III; neurexin III beta; neurexin III alpha)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
INDALHRSGQIERGCEVALTKADLQGPSTTCQEDSCANQG
VCMQQWEGFTCDCSMTSYSGNQCNDPGATYIFGKSGGLIL
YTWPANDRPSTRSDRLAVGF

Other western:
Code: A0608
Lot: 1

Western result: -

GST GST-Neu3 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: Neu3
Full name: Neurexin III
UniGene Number: N.A.
Accession Number: NP_766132
Source organism: mouse ( Mus musculus )
Full length size (aa): 1100
NCBI information page: click here

Full length sequence:
MLGSDDFFYVGGSPSTTDLPGSPVSNNFMGCLKEVVYKNNDIRLELSRLA
RIGDTKMKIYGEVVFKCENVATLDPINFETPEAYISLPKWNTKRMGSISF
DFRTTEPNGLILFTHGKPQERKDVRSQKNTKVDFFAVELLDGNLYLLLDM
GSGTIKVKATQKKANDGEWYHVDIQRDGRSGTISVNSRRTPFTASGESEI
LDLEGDMYLGGLPENRAGLILPTELWTAMLNYGYVGCIRDLFIDGRSKNI
RQLAEMQNAAGVKSSCSRMSAKQCDSYPCKNNAVCKDGWNRFICDCTGTG
YWGRTCEREASILSYDGSMYMKVIMPMVMHTEAEDVSFRFMSQRAYGLLV
ATTSRDSADTLRLELDGGRVKLMVNLDCIRINCNSSKGPETLYAGQKLND
NEWHTVRVVRRGKSLKLTVDDDVAEGTMVGDHTRLEFHNIETGIMTEKRY
ISVVPSSFIGHLQSLMFNGLLYIDLCKNGDIDYCELKARFGLRNIIADPV
TFKTKSSYLTLATLQAYTSMHLFFQFKTTSADGFILFNSGDGNDFIAVEL
VKGYIHYVFDLGNGPNVIKGNSDRPLNDNQWHNVVITRDSSNTHSLKVDT
KVVTQVINGAKNLDLKGDLYMAGLAQGMYSNLPKLVASRDGFQGCLASVD
LNGRLPDLINDALHRSGQIERGCEVALTKADLQGPSTTCQEDSCANQGVC
MQQWEGFTCDCSMTSYSGNQCNDPGATYIFGKSGGLILYTWPANDRPSTR
SDRLAVGFSTTVKDGILVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDI
SIKEERTPVNDGKYHVVRFTRNGGNATLQVDNWPVNEHYPTGNTDNERLQ
MVKQKIPFKYNRPVEEWLQEKGRQLTIFNTQAQIAIGGKDKGRLFQGQLS
GLYYDGLKVLNMAAENNPNIKINGSVRLVGEVPSVSGTTQTTSMPPEMST
TVMETTTTMATTTTRKNRSTASIQPTSDDLVSSAECSSDDEDFVECEPST
ANPTEPGIRRVPGASEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYR
NRDEGSYQVDETRNYISNSAQSNGTLMKEKQASSKSGHKKQKNKDKEYYV