mouse ( Mus musculus )
Nova-1

Western result: +

control Nova-1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: Nova-1
CBI Antibody Core Code: A0606
"Lot" Number: 1 Position: 20(404-423)
% Identical Homology of Target to Mouse Protein: 100% (XP_356586.2| neuro-oncological ventral antigen 1)
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
PLAASAILGTEKSTDGSKDV

Sequence Name: Nova-1
Full name: RNA-binding protein Nova-1 (Neuro-oncological ventral antigen 1)(Ventral neuron-specific protein 1)
UniGene Number: N.A.
Accession Number: Q9JKN6
Source organism: mouse ( Mus musculus )
Full length size (aa): 507
NCBI information page: click here

Full length sequence:
MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQY
FLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVC
LIQGTIEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQT
LPSSPTTTKSSPSDPMTTSRANQVKIIVPNSTAGLIIGKGGATVKAIMEQ
SGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSC
LNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAF
PAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAA
SANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFG
AASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQEL
TGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRA
ANPQKVG