mouse ( Mus musculus )
NR5A1

Antibody Name/Abbreviation: NR5A1
CBI Antibody Core Code: A0718
"Lot" Number: 2 Position: 100(34-133)
% Identical Homology of Target to Mouse Protein: 100% (NP_620639.1| nuclear receptor subfamily 5, group A, member 1; steroidogenic factor 1; adrenal 4-binding protein; fushi tarazu 1 factor homolog)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
KGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKC
LTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQI
RANGFKLETGPPMGVPPPPP

Other western:
Code: A0718
Lot: 1

Western result: +

GST GST-NR5A1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: NR5A1
Full name: nuclear receptor subfamily 5, group A, member 1; fushi tarazu 1 factor homolog; steroidogenic factor 1; adrenal 4-binding protein
UniGene Number: N.A.
Accession Number: NP_620639.1
Source organism: mouse ( Mus musculus )
Full length size (aa): 462
NCBI information page: click here

Full length sequence:
MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCT
ESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYK
RDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPPPDYMLPPSLHAPEPK
ALVSGPPSGPLGDFGAPSLPMAVPGPHGPLAGYLYPAFSNRTIKSEYPEP
YASPPQQPGPPYSYPEPFSGGPNVPELILQLLQLEPEEDQVRARIVGCLQ
EPAKSRSDQPAPFSLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQ
NCWSELLVLDHIYRQVQYGKEDSILLVTGQEVELSTVAVQAGSLLHSLVL
RAQELVLQLHALQLDRQEFVCLKFLILFSLDVKFLNNHSLVKDAQEKANA
ALLDYTLCHYPHCGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRN
NLLIEMLQAKQT