mouse ( Mus musculus )
P39-C

Western result: +

control P39-N (fragment)

29.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: P39-N
CBI Antibody Core Code: A0507
"Lot" Number: 1 Position: 20(98-117)
% Identical Homology of Target to Mouse Protein: 100% (NP_034002.1| cyclin-dependent kinase 5, regulatory subunit 2 (p39))
Molecular Weight of GST-fusion: 29.2 KDa

Target sequence used to make this antibody:
QRNRENLLRKGRDGPDGGGT

Sequence Name: P39-C
Full name: cyclin-dependent kinase 5, regulatory subunit 2
UniGene Number: N.A.
Accession Number: NP_034002
Source organism: mouse ( Mus musculus )
Full length size (aa): 369
NCBI information page: click here

Full length sequence:
MGTVLSLSPASSAKGRRPGGLPEEKKKAPPAGDEALGGYGAPPAGKGGKG
ESRLKRPSVLISALTWKRLVAASAKKKKGSKKVTPKPASTGPDPLVQQRN
RENLLRKGRDGPDGGGTAKPLAVPVPTVPTTAATCEPPSGGSAAAPPPGS
GGGKPPPPPPPAPQAAPPAPGSSPRRVIVQASTGELLRCLRDFVCRSCYR
LKELSPGELVGWFRGVDRSLLLQGWQDQAFITPANLVFVYLLCRESLSGD
ELASAAELQAAFLTCLYLAYSYMGNEISYPLKPFLVEPDKERFWQRCLRL
IQRLSPQMLRLNADPHFFTQVFQDLKNEGEAAASTGGPPSGSSASTTSSS
SARDSCATGAKHWTMNLDR