mouse ( Mus musculus )
TRAP100

Western result: +

GST GST-TRAP100 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TRAP100
CBI Antibody Core Code: A0767
"Lot" Number: 3 Position: 100 (308-407)
% Identical Homology of Target to Mouse Protein: 100% (NP_035999.1| thyroid hormone receptor associated protein 4; peroxisome proliferation activation receptor binding protein 2; thyroid hormone receptor-associated protein 100 kDa; thyroid hormone receptor-associated protein 100; genetic suppressor element 2)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
EFLLKLTPLLDKADQRCNCDCTNFLLQECNKQGLLSEVNF
ASLVGKRTADRDPQLKSSENANIQPNPGLILRAEPTVTNI
LKTMDADHSKSPEGLLGVLG

Other western:
Code: A0767
Lot: 2

Western result: +

GST GST-TRAP100 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: TRAP100
Full name: gi|5359714|gb|AAD42776.1|AF126543_1 100 kDa thyroid hormone receptor associated
UniGene Number: N.A.
Accession Number: AF126543; GI:5359713
Source organism: mouse ( Mus musculus )
Full length size (aa): 956
NCBI information page: click here

Full length sequence:
MIGPSPNPLILSYLKYAISSQMVSCSSVLTAISKFDDFSRDLCVQALLDI
MDMFCDRLSCHGKAEECIGLCRALLSALHWLLRCTAASAERLQEGLEAGT
PAPGEKQLALCLQCLEKTLSSTKNRALLHIAKLEEASLHTSQGLGQGGTR
ANQPTASWTAIEHSLLKLGEILANLSNPQLRSQAERCGTLIRSIPSMLSV
HSEQLHKTGFPTIHALILLEGTMNLTGEMQPLVEQLMMVKRMQHIPTPLF
VLEIWKACFVGLIESPEGTQELKWTAFTYLKIPQVLVKLKKYFHGEKDFT
EDVNCAFEFLLKLTPLLDKADQRCNCDCTNFLLQECNKQGLLSEVNFASL
VGKRTADRDPQLKSSENANIQPNPGLILRAEPTVTNILKTMDADHSKSPE
GLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTHGSGEST
KTASVRALLFDISFLMLCHVAHTYGSEVILSESSSGEEVPFFETWMQTCM
PEEGKILNPEHPCFRPDSTKVESLVALLNNSSEMNLVQMKWHEACLSISA
AILEILNAWENGVLAFESIQKITDNINGKVCSLAVCAVAWLVAHVRMLGL
DEREKSLQMIRQLAGPLYSENTLQFYNERVVIMNSILEHMCADVLQQTAT
QIKFPSTGVDTMPYWNLFPPKRPIKEVLTDIFAKVLEKGWVDSRSIHILD
TLLHMGGVYWFCNNLIKELLKETRKEHTLRAVQLLYSIFCLDMQQVTLVL
LGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASSRQK
KRHREDIEDYVSLFPVEDMQPSKLMRLLSSSDDDANILSSPTDRSMNSSL
SASQLHRVNMRDPLNRVLANLFLLISSILGSRTAGPHTQFVQWFMEECVG
CLEQDSRGTILQFMPFTTVSELVKVSAMSSPEVVLAITHLSLPLGRQVAA
KAIAAL