mouse ( Mus musculus )
TrkB

Antibody Name/Abbreviation: TrkB
CBI Antibody Core Code: A0727
"Lot" Number: 2 Position: 100(334-433)
% Identical Homology of Target to Mouse Protein: 100% (NP_032771.1| neurotrophic tyrosine kinase, receptor, type 2)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
IHVTNHTEYHGCLQLDNPTHMNNGDYTLMAKNEYGKDERQ
ISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKS
NEIPSTDVADQSNREHLSVY

Other western:
Code: A0727
Lot: 1

Western result: +

GST GST-TrkB (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: TrkB
Full name: brain-derived neurotrophic factor receptor precursor
UniGene Number: N.A.
Accession Number: S06943
Source organism: mouse ( Mus musculus )
Full length size (aa): 821
NCBI information page: click here

Full length sequence:
MSPWLKWHGPAMARLWGLCLLVLGFWRASLACPTSCKCSSARIWCTEPSP
GIVAFPRLEPNSVDPENITEILIANQKRLEIINEDDVEAYVGLRNLTIVD
SGLKFVAYKAFLKNSNLRHINFTRNKLTSLSRRHFRHLDLSDLILTGNPF
TCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNMPLANLQIPNCGLPSAR
LAAPNLTVEEGKSVTLSCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSL
RITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHH
WCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDN
PTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDW
TTPTDIGDTTNKSNEIPSTDVADQSNREHLSVYAVVVIASVVGFCLLVML
LLLKLARHSKFGMKGPASVISNDDDSASPLHHISNGSNTPSSSEGGPDAV
IIGMTKIPVIENPQYFGITNSQLKPDTFVQHIKRHNIVLKRELGEGAFGK
VFLAECYNLCPEQDKILVAVKTLKDASDNARKDFHREAELLTNLQHEHIV
KFYGVCVEGDPLIMVFEYMKHGDLNKFLRAHGPDAVLMAEGNPPTELTQS
QMLHIAQQIAAGMVYLASQHFVHRDLATRNCLVGENLLVKIGDFGMSRDV
YSTDYYRVGGHTMLPIRWMPPESIMYRKFTTESDVWSLGVVLWEIFTYGK
QPWYQLSNNEVIECITQGRVLQRPRTCPQEVYELMLGCWQREPHTRKNIK
SIHTLLQNLAKASPVYLDILG