mouse ( Mus musculus )
TRIF

Western result: +

GST GST-TRIF (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: TRIF
CBI Antibody Core Code: A0313
"Lot" Number: 1 Position: 100(86-185)
% Identical Homology of Target to Mouse Protein: 64% (NP_778154.1| TICAM-1; TIR domain-containing adaptor inducing interferon-beta)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
PEEPPDVSWAVARLYHLLAEEKLCPASLRDVAYQEAVRTL
SSRDDHRLGELQDEARNRCGWDIAGDPGSIRTLQSNLGCL
PPSSALPSGTRSLPRPIDGV

Sequence Name: TRIF
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: mouse ( Mus musculus )
Full length size (aa): 712
NCBI information page:

Sequence Notes: TRIF is TRIF-N. Also available: TRIF-C

Full length sequence:
MACTGPSLPSAFDILGAAGQDKLLYLKHKLKTPRPGCQGQDLLHAMVLLK
LGQETEARISLEALKADAVARLVARQWAGVDSTEDPEEPPDVSWAVARLY
HLLAEEKLCPASLRDVAYQEAVRTLSSRDDHRLGELQDEARNRCGWDIAG
DPGSIRTLQSNLGCLPPSSALPSGTRSLPRPIDGVSDWSQGCSLRSTGSP
ASLASNLEISQSPTMPFLSLHRSPHGPSKLCDDPQASLVPEPVPGGCQEP
EEMSWPPSGEIASPPELPSSPPPGLPEVAPDATSTGLPDTPAAPETSTNY
PVECTEGSAGPQSLPLPILEPVKNPCSVKDQTPLQLSVEDTTSPNTKPCP
PTPTTPETSPPPPPPPPSSTPCSAHLTPSSLFPSSLESSSEQKFYNFVIL
HARADEHIALRVREKLEALGVPDGATFCEDFQVPGRGELSCLQDAIDHSA
FIILLLTSNFDCRLSLHQVNQAMMSNLTRQGSPDCVIPFLPLESSPAQLS
SDTASLLSGLVRLDEHSQIFARKVANTFKPHRLQARKAMWRKEQDTRALR
EQSQHLDGERMQAAALNAAYSAYLQSYLSYQAQMEQLQVAFGSHMSFGTG
APYGARMPFGGQVPLGAPPPFPTWPGCPQPPPLHAWQAGTPPPPSPQPAA
FPQSLPFPQSPAFPTASPAPPQSPGLQPLIIHHAQMVQLGLNNHMWNQRG
SQAPEDKTQEAE