mouse ( Mus musculus )
HSF4-FL

Western result: +

GST GST-HSF4 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: HSF4
CBI Antibody Core Code: A0933
"Lot" Number: 1 Position: 100(242-341)
% Identical Homology of Target to Mouse Protein: 80% (NP_036069.1| heat shock transcription factor 4)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
QSPSPCSPSQRPRWASALTGPEGPSSLTSQKILHLLKDTG
FLPPVVAGAPPPLPVAVVQAILEGKGSYSPEGPRSVQQPE
PRGPREVPDRGTLGLDRGNR

Sequence Name: HSF4-FL
Full name:
UniGene Number: N.A.
Accession Number: NM_011938
Source organism: mouse ( Mus musculus )
Full length size (aa): 462
NCBI information page:

Full length sequence:
MQEAPAALPTEPGPSPVPAFLGKLWALVGDPGTDHLIRWSPSGTSFLVSD
QSRFAKEVLPQYFKHSNMASFVRQLNMYGFRKVVSIEQGGLLRPERDHVE
FQHPSFVRGREQLLERVRRKVPALRGDDSRWRPEDLSRLLGEVQALRGVQ
ESTEARLQELRQQNEILWREVVTLRQSHSQQHRVIGKLIQCLFGPLQTGP
SSTGAKRKLSLMLDEGSACSASAKFNACPVSGALLQDPYFIQSPSPCSPS
QRPRWASALTGPEGPSSLTSQKILHLLKDTGFLPPVVAGAPPPLPVAVVQ
AILEGKGSYSPEGPRSVQQPEPRGPREVPDRGTLGLDRGNRSPESLLPPM
LLRPAPETLEPVAPVDVLGPSLHGREWTLMDLDMELSLMQPLAPETDEAE
LTVKELNSSGVGKDHTLGTPLMLDVQADLEGAALSVPGALTLYNVTESNA
SYLDPGASPSSP