mouse ( Mus musculus )
galc

Western result: +/-

GST GST-galc (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: galc
CBI Antibody Core Code: A0947
"Lot" Number: 1 Position: 100 (428-527)
% Identical Homology of Target to Mouse Protein: 88% (NP_032105.1| galactosylceramidase; galactocerebrosidase)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
TLWLLDGSGSFTLELEEDEIFTLTTLTTGRKGSYPPPPSS
KPFPTNYKDDFNVEYPLFSEAPNFADQTGVFEYYMNNEDR
EHRFTLRQVLNQRPITWAAD

Sequence Name: galc
Full name: galactosylceramidase
UniGene Number: N.A.
Accession Number: NP_032105
Source organism: mouse ( Mus musculus )
Full length size (aa): 668
NCBI information page:

Full length sequence:
mtaaagsasrvavplllcallvpggayvlddsdglgrefdgigavsggga
tsrllvnypepyrseildylfkpnfgaslhilkveiggdgqttdgtepsh
mhyeldenyfrgyewwlmkeakkrnpdiilmglpwsfpgwlgkgfswpyv
nlqltayyvvrwilgakhyhdldidyigiwnerpfdanyikelrkmldyq
glqrvriiasdnlwepisssllldqelwkvvdvigahypgtytvwnakms
gkklwssedfstinsnvgagcwsrilnqnyingnmtstiawnlvasyyee
lpygrsglmtaqepwsghyvvaspiwvsahttqftqpgwyylktvghlek
ggsyvaltdglgnltiiietmshqhsmcirpylpyynvshqlatftlkgs
lreiqelqvwytklgtpqqrlhfkqldtlwlldgsgsftleleedeiftl
ttlttgrkgsyppppsskpfptnykddfnveyplfseapnfadqtgvfey
ymnnedrehrftlrqvlnqrpitwaadasstisvigdhhwtnmtvqcdvy
ietprsggvfiagrvnkggilirsatgvffwifangsyrvtadlggwity
asghadvtakrwytltlgikgyfafgmlngtilwknvrvkypghgwaaig
thtfefaqfdnfrveaar