Streptococcus pneumoniae TIGR4
SP2192 (spSHK)

Western result: +

GST GST-SP2192 (spSHK) (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: SP2192 (spSHK)
CBI Antibody Core Code: A0359
"Lot" Number: 1 Position: 100(344-443)
% Identical Homology of Target to Mouse Protein: 35% (XP_127913.3| RIKEN cDNA 4930455B06)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
RALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCA
PLSDQELEHLFDIFYHSQIVTDKDESSGLGLYIVNNILES
YQMDYSFLPYEHGMEFKISL

Sequence Name: SP2192 (spSHK)
Full name: Senso histidine kinase
UniGene Number: N.A.
Accession Number: AAK76243
Source organism: Streptococcus pneumoniae TIGR4
Full length size (aa): 443
NCBI information page: click here

Full length sequence:
MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGEKFNESA
RVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIK
DGKLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFL
VTIAFSFVFSYFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGE
VGKQINGMYEHLLKVIYELESRNEQIVKLQNQKVSFVRGASHELKTPLAS
LRIILENMQHNIGDYKDHPKYIAKSINKIDQMSHLLEEVLESSKFQEWTE
CRETLTVKPVLVDILSRYQELAHSIGVTIENQLTDATRVVMSLRALDKVL
TNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEHLFDIFYHS
QIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL