mouse adenovirus type 1 (MAV-1)
MAV100K

Western result: +

GST GST-MAV100K (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: MAV100K
CBI Antibody Core Code: A0769
"Lot" Number: 2 Position: 100(613-712)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
RREFLHQKGRGVYLDPQTGEEIGKGSADPASLSDSDSHAE
DREGQPTAGAGRRGRVERGRVQRRQRQRPGGHFIRGGRSS
PDGCSAPARERNRENEPPAL

Other western:
Code: A0769
Lot: 1

Western result: +

GST GST-MAV100K (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: MAV100K
Full name: gi|33694692|tpg|DAA00691.1| TPA: 100K [Murine adenovirus 1]
UniGene Number: N.A.
Accession Number: DAA00691; GI: 33694692
Source organism: mouse adenovirus type 1 (MAV-1)
Full length size (aa): 743
NCBI information page: click here

Full length sequence:
MQSNTQESETTEVPDTNPTPLEEETSKSDTHEATNDSNYLSADSLLVHIQ
RQARILREALQPEQDKTEWSATTLSHMLERELFTRSNAKFPAVPKQSNGS
MSPDPRLNFYPAFMVPEALATYHPFFVNQRIPLSCRANRPLADKNWELKD
GSRLPDIVTVQEQPRFFESLQGQELSRNQLEVAGSDSETGVLTELEGDSP
RLAVVKRCLSVTHAAYPALGLPPKVSKIMSEELTFKTNLETGDNSQPVVS
DEQLQKWLKAKDPADPAIEEHRKRVMAVTLITCQLEALRSFFSRADVIRR
VGESLHYLFQHGYVKQAIQVCQTDLTHLVTYMGIVHENRLGQTVLHGSLL
GEARRDYIRDTVYLFLIYTWQTAMGVWQQCLEDENLEKLRKILQSHRRSL
YTETNERLLAAELRRLVFPAHLENTLAQGLPDILHQSIINNFRSFILERS
GILPATTCAYPSDFVPTTFKECPPPLWAHTYLLTLANYFMYHNDLQEDLT
GAGLLSAHCRCNLCSPHRSLVHNTALLNEVKCINTFELQGPPGKDGKSGP
CFKLTPGLWVSAYLRKFEEQDYHPYSITYFENSKKQQPKTPLSACVLTQT
ELVAQLLAINDHRREFLHQKGRGVYLDPQTGEEIGKGSADPASLSDSDSH
AEDREGQPTAGAGRRGRVERGRVQRRQRQRPGGHFIRGGRSSPDGCSAPA
RERNRENEPPALECDCHHHHHHKEEEKESTHSMGLSSSTEHIG