SARS
SARS-Nsp1

Western result: +-

GST GST-SARS-Nsp1 (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: SARS-Nsp1
CBI Antibody Core Code: A0058
"Lot" Number: 1 Position: 101()
% Identical Homology of Target to Mouse Protein: 42% (XP_194369.3| similar to X-ray radiation resistance associated 1 protein)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
KRVMFNGVTFSTFEEAALCTFLLNKEMYLKLRSETLLPLT
QYNRYLALYNKYKYFSGALDTTSYREAACCHLAKALNDFS
NSGADVLYQPPQTSITSAVLQ

Sequence Name: SARS-Nsp1
Full name:
UniGene Number: N.A.
Accession Number: NC004718 (c)
Source organism: SARS
Full length size (aa): 2422
NCBI information page: click here

Full length sequence:
GAPIKGVTFGEDTVWEVQGYKNVRITFELDERVDKVLNEKCSVYTVESGT
EVTEFACVVAEAVVKTLQPVSDLLTNMGIDLDEWSVATFYLFDDAGEENF
SSRMYCSFYPPDEEEEDDAECEEEEIDETCEHEYGTEDDYQGLPLEFGAS
AETVRVEEEEEEDWLDDTTEQSEIEPEPEPTPEEPVNQFTGYLKLTDNVA
IKCVDIVKEAQSANPMVIVNAANIHLKHGGGVAGALNKATNGAMQKESDD
YIKLNGPLTVGGSCLLSGHNLAKKCLHVVGPNLNAGEDIQLLKAAYENFN
SQDILLAPLLSAGIFGAKPLQSLQVCVQTVRTQVYIAVNDKALYEQVVMD
YLDNLKPRVEAPKQEEPPNTEDSKTEEKSVVQKPVDVKPKIKACIDEVTT
TLEETKFLTNKLLLFADINGKLYHDSQNMLRGEDMSFLEKDAPYMVGDVI
TSGDITCVVIPSKKAGGTTEMLSRALKKVPVDEYITTYPGQGCAGYTLEE
AKTALKKCKSAFYVLPSEAPNAKEEILGTVSWNLREMLAHAEETRKLMPI
CMDVRAIMATIQRKYKGIKIQEGIVDYGVRFFFYTSKEPVASIITKLNSL
NEPLVTMPIGYVTHGFNLEEAARCMRSLKAPAVVSVSSPDAVTTYNGYLT
SSSKTSEEHFVETVSLAGSYRDWSYSGQRTELGVEFLKRGDKIVYHTLES
PVEFHLDGEVLSLDKLKSLLSLREVKTIKVFTTVDNTNLHTQLVDMSMTY
GQQFGPTYLDGADVTKIKPHVNHEGKTFFVLPSDDTLRSEAFEYYHTLDE
SFLGRYMSALNHTKKWKFPQVGGLTSIKWADNNCYLSSVLLALQQLEVKF
NAPALQEAYYRARAGDAANFCALILAYSNKTVGELGDVRETMTHLLQHAN
LESAKRVLNVVCKHCGQKTTTLTGVEAVMYMGTLSYDNLKTGVSIPCVCG
RDATQYLVQQESSFVMMSAPPAEYKLQQGTFLCANEYTGNYQCGHYTHIT
AKETLYRIDGAHLTKMSEYKGPVTDVFYKETSYTTTIKPVSYKLDGVTYT
EIEPKLDGYYKKDNAYYTEQPIDLVPTQPLPNASFDNFKLTCSNTKFADD
LNQMTGFTKPASRELSVTFFPDLNGDVVAIDYRHYSASFKKGAKLLHKPI
VWHINQATTKTTFKPNTWCLRCLWSTKPVDTSNSFEVLAVEDTQGMDNLA
CESQQPTSEEVVENPTIQKEVIECDVKTTEVVGNVILKPSDEGVKVTQEL
GHEDLMAAYVENTSITIKKPNELSLALGLKTIATHGIAAINSVPWSKILA
YVKPFLGQAAITTSNCAKRLAQRVFNNYMPYVFTLLFQLCTFTKSTNSRI
RASLPTTIAKNSVKSVAKLCLDAGINYVKSPKFSKLFTIAMWLLLLSICL
GSLICVTAAFGVLLSNFGAPSYCNGVRELYLNSSNVTTMDFCEGSFPCSI
CLSGLDSLDSYPALETIQVTISSYKLDLTILGLAAEWVLAYMLFTKFFYL
LGLSAIMQVFFGYFASHFISNSWLMWFIISIVQMAPVSAMVRMYIFFASF
YYIWKSYVHIMDGCTSSTCMMCYKRNRATRVECTTIVNGMKRSFYVYANG
GRGFCKTHNWNCLNCDTFCTGSTFISDEVARDLSLQFKRPINPTDQSSYI
VDSVAVKNGALHLYFDKAGQKTYERHPLSHFVNLDNLRANNTKGSLPINV
IVFDGKSKCDESASKSASVYYSQLMCQPILLLDQALVSDVGDSTEVSVKM
FDAYVDTFSATFSVPMEKLKALVATAHSELAKGVALDGVLSTFVSAARQG
VVDTDVDTKDVIECLKLSHHSDLEVTGDSCNNFMLTYNKVENMTPRDLGA
CIDCNARHINAQVAKSHNVSLIWNVKDYMSLSEQLRKQIRSAAKKNNIPF
RLTCATTRQVVNVITTKISLKGGKIVSTCFKLMLKATLLCVLAALVCYIV
MPVHTLSIHDGYTNEIIGYKAIQDGVTRDIISTDDCFANKHAGFDAWFSQ
RGGSYKNDKSCPVVAAIITREIGFIVPGLPGTVLRAINGDFLHFLPRVFS
AVGNICYTPSKLIEYSDFATSACVLAAECTIFKDAMGKPVPYCYDTNLLE
GSISYSELRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDAEYCRHGTCE
RSEVGICLSTSGRWVLNNEHYRALSGVFCGVDAMNLIANIFTPLVQPVGA
LDVSASVVAGGIIAILVTCAAYYFMKFRRVFGEYNHVVAANALLFLMSFT
ILCLVPAYSFLPGVYSVFYLYLTFYFTNDVSFLAHLQWFAMFSPIVPFWI
TAIYVFCISLKHCHWFFNNYLRKRVMFNGVTFSTFEEAALCTFLLNKEMY
LKLRSETLLPLTQYNRYLALYNKYKYFSGALDTTSYREAACCHLAKALND
FSNSGADVLYQPPQTSITSAVLQ