West Nile Virus (WNV)
WNV-NS2B

Western result: +

control WNV-NS2B (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: WNV-NS2B
CBI Antibody Core Code: A0092b
"Lot" Number: 1 Position: 20 (77-96)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
VRLDDDGNFQLMNDPGAPWK

Sequence Name: WNV-NS2B
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: West Nile Virus (WNV)
Full length size (aa): 131
NCBI information page:

Full length sequence:
GWPATEVMTAVGLMFAIVGGLAELDIDSMAIPMTIAGLMFAAFVISGKST
DMWIERTADISWESDAEITGSSERVDVRLDDDGNFQLMNDPGAPWKIWML
RMVCLAISAYTPWAILPSVVGFWITLQYTKR