marine platyhelminth ( Macrostomum sp. )
MCM

Western result: +

GST GST-MacMCM (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: MacMCM
CBI Antibody Core Code: A1027a
"Lot" Number: 1 Position: 100 (182-281)
% Identical Homology of Target to Mouse Protein: 66% (NP_032590.1| minichromosome maintenance deficient 2 mitotin; mini chromosome maintenance deficient 2 (S. cerevisiae))
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
GPFLQSQCCGATENRPVSCPECQTGGPFELNSEQTVYKNY
QRVSLQESPGSVPPGRLPRAKDVILLEDLVDSCKPGDEVE
LTAIYTHSYESSLNSAHGFP

Sequence Name: MCM
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: marine platyhelminth ( Macrostomum sp. )
Full length size (aa): 781
NCBI information page:

Full length sequence:
AAAFLMDDEGEGMDALENLENTRGMSIAEWVKQPQARHEVKNRFMNFLRS
TTDSAGQNVYQQRIIAMCQANKASLVVDYVDLAAREHVLAYFLPEAPIEM
LQILDEAAMAVVRGAVANYDRIQPIIRVRVSGLPLLEQLRQLRHSHLNQL
VRTRGVVTSCSSVLPQLSLAKYNCTKCGSVIGPFLQSQCCGATENRPVSC
PECQTGGPFELNSEQTVYKNYQRVSLQESPGSVPPGRLPRAKDVILLEDL
VDSCKPGDEVELTAIYTHSYESSLNSAHGFPVFSTVLHANHVLKKEDKMA
AEALTEEDIRTINRLAKDGRIGDRIVNSIAPSIYGHEDIKRAIALCLFGG
EAKNPGGKHRLRGDINLLVCGDPGTAKSQFLKYCEGIAPRCVYATGQGAS
SVGLTAYVHKHPVTKEWCLEGGALVLADKGVCLIDEFDKMSDSDRTSIHE
AMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSMTFAENVDLTEP
ILSRFDVLCVVRDQVDPVQDEKLARFVVGSHMRHHPCLSEAERQQLADSL
AADRNSSIADSSLEPLPQDLLKKYITYARERIHPQLNQMDQEKVSKVYVH
LRKESMATGSIPITVRHIESVIRLAEAHAKMHLRKYVDDSDVNMAVRVML
ESFISTQKHSVMKTMERSFRRYLVYQRDSGDLLVFLLRQLIHKHAAYSRT
SRRARPGQRAAGAAADSDDDATPDDDDAEGPLTVRIAEKELADLARPLGI
TDLDGFFRSEIMTANRFRYDADRREIVCQVY