Molluscum contagiosum virus (MCV) ( Molluscum contagiosum )
MC159L

Western result: +/-

GST GST-MC159L (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: MC159L
CBI Antibody Core Code: A0952
"Lot" Number: 1 Position: 100 (142-241)
% Identical Homology of Target to Mouse Protein: 29% (XP_138810.3| similar to zinc finger protein 366)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
ALENVGLVSPSSVSVLADMLRTLRRLDLCQQLVEYEQQEQ
ARYRYCYAASPSLPVRTLRRGHGASEHEQLCMPVQESSDS
PELLRTPVQESSSDSPEQTT

Sequence Name: MC159L
Full name: MC159L
UniGene Number: N.A.
Accession Number: NC_001731
Source organism: Molluscum contagiosum virus (MCV) ( Molluscum contagiosum )
Full length size (aa): 241
NCBI information page: click here

Full length sequence:
MSDSKEVPSLPFLRHLLEELDSHEDSLLLFLCHDAAPGCTTVTQALCSLS
QQRKLTLAALVEMLYVLQRMDLLKSRFGLSKEGAEQLLGTSFLTRYRKLM
VCVGEELDSSELRALRLFACNLNPSLSTALSESSRFVELVLALENVGLVS
PSSVSVLADMLRTLRRLDLCQQLVEYEQQEQARYRYCYAASPSLPVRTLR
RGHGASEHEQLCMPVQESSDSPELLRTPVQESSSDSPEQTT