ECTV (ectromelia virus Moscow strain)
EVM138

Western result: +

GST GST-EVM138 (fragment)

24.01 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: EVM138
CBI Antibody Core Code: A1165
"Lot" Number: 1 Position: 91 (31-121)
% Identical Homology of Target to Mouse Protein: 29% (NP_034711.1| CD47 antigen; integrin-associated protein; Rh-related antigen)
Molecular Weight of fusion protein: 24.01 KDa

Target sequence used to make this antibody:
TIIIPCTIDNPTKYIRWKLDNHDILTYNKTSKTTILSKWH
TSARLHSLSDNDVSLIMEYKDILPGTYTCGDNTGIKSTVK
LVQCHTNWFND

Sequence Name: EVM138
Full name: EVM138
UniGene Number: N.A.
Accession Number: AAM92443
Source organism: ECTV (ectromelia virus Moscow strain)
Full length size (aa): 277
NCBI information page:

Full length sequence:
MLRVRISLIYLYTLVVITTTKTIEYTACNDTIIIPCTIDNPTKYIRWKLD
NHDILTYNKTSKTTILSKWHTSARLHSLSDNDVSLIMEYKDILPGTYTCG
DNTGIKSTVKLVQCHTNWFNDYQTTLMFIFTGITLFLLFLEIAYTSISVV
FSTNLGILQVFGCVIAMIELCGAFLFYPSMFTLRHIIGLLMITLPSIFLI
ITKVFSFWLLCKLSCAVHLIIYYQLAGYILTVLGLGLSLKECVDGTLLLS
GLGTIMVSEHFSLLFLVYFPSTQRDYY