Campylobacteriosis (C. jejuni) ( Campylobacter jejuni )
cj1190c

Western result: +

GST GST-cetA A1172a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: cetA
CBI Antibody Core Code: A1172a
"Lot" Number: 1 Position: 100 (355-454)
% Identical Homology of Target to Mouse Protein: 34% (XP_146948.4| unc13 homolog 3)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
SVVKIDHILYKSNIYLNLNGAQNFNLESVDPISNLCQDER
AQGVINELSSETELNLAKEFIKDNAKKAIEESSQDYIDQK
AYDAIVNDIKSLEQRSAEIL

Antibody Name/Abbreviation: cetA
CBI Antibody Core Code: A1172b
"Lot" Number: 1 Position: 20 (161-180)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 32.2 KDa

Target sequence used to make this antibody:
LSLGNQNKDMSQISSSLNQD

Sequence Name: cj1190c
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: Campylobacteriosis (C. jejuni) ( Campylobacter jejuni )
Full length size (aa): 459
NCBI information page:

Full length sequence:
MVKNGLLLTIFLSVIGVLGVIFIHIFVGAIIFVLIAVLMIYLLRQHKDEQ
IMIDKLLVLCRELKEGNFDNRIIYVKTKSKKLAEIADNLNNTIDGLEAYL
REINTSISCSQKGEFYRKALPEGLKGIFAHNIEFINKALANIEVTARSTF
KNALSRTLMDLSLGNQNKDMSQISSSLNQDISMMKNVYDTVDAISHTATE
NGSEVDSLQSAMGSLMDVVNSSKETVQTFVANSQNITSVVEVIRDIADQT
NLLALNAAIEAARAGEHGRGFAVVADEVRKLAERTQRSTSEISIAIQTMQ
QDFVNIQSGSEQVFNIVSESEERINKFSQAFKRLEENSSALGVNFGSFAK
RLILSVVKIDHILYKSNIYLNLNGAQNFNLESVDPISNLCQDERAQGVIN
ELSSETELNLAKEFIKDNAKKAIEESSQDYIDQKAYDAIVNDIKSLEQRS
AEILAKLKI