Q fever ( Coxiella burnetii )
20R

Western result: +

GST GST-20R (fragment)

18.62 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: 20R
CBI Antibody Core Code: A1215
"Lot" Number: 1 Position: 42 (310-351)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 18.62 KDa

Target sequence used to make this antibody:
QHGGQHALEFFTSKTIGNPNVNTENEDVEAAAPSVSGTIG
YT

Sequence Name: 20R
Full name:
UniGene Number: N.A.
Accession Number: CBU0970
Source organism: Q fever ( Coxiella burnetii )
Full length size (aa): 351
NCBI information page:

Full length sequence:
MNKKISLFLSLSIALTSGLTFYSAAAEETLDVQFLILAIVVLFSEFFEIK
DHLEHTHDHHDAARKPLLPLPGIDPPNNYSSTNQSQTTNVLLNRTSTIST
IVKGSATICTLPFFGFACYGLWCQSKSLMDFFNISTKLTFIPAIFLWGRY
ALIPVSHTIRNIWSQEGVEKFTARVTHTAERKGFIQNKAVLFFTFLGLSG
THLPESFLLSNKTKGWKEILSILGLSIPRAIAHLDHVRALPQTLKEVWQK
NRKGKLALLCFAACGAGLIHASPALLSTFYYWDKTDIELKIALGTTVGIE
TLTGAGEFLQHGGQHALEFFTSKTIGNPNVNTENEDVEAAAPSVSGTIGY
T