Chinese hamster ( Cricetulus griseus )
ERCC1

Western result: +

GST GST-ERCC1 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: ERCC1
CBI Antibody Core Code: A1229
"Lot" Number: 1 Position: 100 (9-108)
% Identical Homology of Target to Mouse Protein: 62% (NP_031974.1| excision repair cross-complementing rodent repair deficiency, complementation group 1; excision repair 1)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
SLPQPTRKKFVIPLEDEAPPAGAKPLFRSSRNPSTTAPSV
PAAPQTYAEYAIAQPPGGAGPTGPTGSEPVKGENPGQTVK
TGAKSNSILVSPRQRGNPVL

Sequence Name: ERCC1
Full name:
UniGene Number: N.A.
Accession Number: AB008427.1
Source organism: Chinese hamster ( Cricetulus griseus )
Full length size (aa): 293
NCBI information page: click here

Full length sequence:
MDLGKDEGSLPQPTRKKFVIPLEDEAPPAGAKPLFRSSRNPSTTAPSVPA
APQTYAEYAIAQPPGGAGPTGPTGSEPVKGENPGQTVKTGAKSNSILVSP
RQRGNPVLKFVRNVPWEFGEVTPDYVLGQSTCALFLSLRYHNLHPDYIHE
RLQSLGKSFALRVLLVQVDVKDPQKALKDLAKMCILADCTLVLAWSAEEA
GRYLETYKAYEQKPADLLMEKLEQNFLSRATECLTTVKSVNKTDSQTLLA
TFGSLEQLLTASREDLALCPGLGPQKARRLFDVLHEPFLKVPR