elegant worm (C. elegans) ( Caenorhabditis elegans )
T26C12.4

Western result: +

GST GST-T26C12.4 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: T26C12.4
CBI Antibody Core Code: A1212
"Lot" Number: 1 Position: 100 (821-920)
% Identical Homology of Target to Mouse Protein: 41% (NP_776149.1| natriuretic peptide receptor 2; Nppb receptor; guanylyl cyclase-B)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
MDYIRDGTKTFRPTVHDRTQIHPDLVALLLDCWNENPEVR
PSIRRVRLNTENYLKVKGSLVDQMMRMMEQYANNLEKLVA
ERTGMLEEANVRADKLLGQL

Sequence Name: T26C12.4
Full name:
UniGene Number: N.A.
Accession Number: N.A.
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 1139
NCBI information page:

Full length sequence:
MPNAGFWSAAYAEMPISKFTAYAENAVMPKNSECRTGISLSIGSYHEISE
EGFKIWDNPTGVGPPLRLIAKIYTHFFAFRYFQCVVKVGHIGAVGSMRNA
EKILQLSKEQLTQEGVLGNDFDIEILNQMGCGESYEGVAVGADMYHVQGV
RAFIGPYCNAELDAVAKMATFWNIPVVGYMASSNSFADKTIFKTLARVSL
RTTNSLAEAAAALIKHYGWNKVAIATNTGAVAFERVQSFEEVFHQRGINV
VRKIMLEEYTNAKAIMNSGLLQELENSARVVVCAFSSTRDMNKEFMQAVT
LSGMNNANYAWILPWLQLETKDMAPWLGENGEYQQNVKDHFANSFIIDDV
NGFDNTLVTPFKERLEASGYSTDDLEMKNIYGYIHLYDALRLYALAVRAT
MNETGNENSYLNGKEVWNHMRRITFPGLVSNAGVTSGTVMMDDIAERAPV
YAAFYVPPNSDTVRKVCELEPVMLTNCDGTKTGNGCYELQVTDLSTGFWP
SIDGSLPADEPACGFRNEKCDYTTLIIGGCIVLLIILLIICFFILSRVCE
NRALANTPWRIYRDDFRTIQENEMKSMLSIGSSKTKMSNMSMFVKHHAVV
GTNTHASFHLYPQRRPIVFNRQDLQLLNQMKQAVHDNLNPFLGMSFNEKE
EMVVLWKFCSRGTIQDMIYNQEVSLDSKFHGAFIRDITLGLEYLHSSIIG
YHGSLTPWSCLIDRNWMIKLTDYGIANPLERWEKLGLISTETLKEGDDKS
GSAQKTSLLYQPPEMLKNKESNRTRRMDQSWVKQSQARRQMGDIYAFGMV
MHEILFRALPFPNGTNVSEVMDYIRDGTKTFRPTVHDRTQIHPDLVALLL
DCWNENPEVRPSIRRVRLNTENYLKVKGSLVDQMMRMMEQYANNLEKLVA
ERTGMLEEANVRADKLLGQLLPKYVANELKMGRSVPAKTFDMATVMFSDI
VGFTTICSSSTPLEVVSMLNSIYSKFDDAINKHGSYKVETIGDAYMIVSG
IPEENGNEHIRNICNTALELMLLLKTYEIPHRRNVKLRIRLGIHTGTVAA
GVVGLTAPRYCLFGDTVNVASRMESTSEPEKIQMSQEARDFCVRYYSEFQ
ITLRGTVEAKGKGPVTSYWLLGKQSESQMQQQNFSQLGI