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elegant worm (C. elegans) ( Caenorhabditis elegans )
CXXC1


Antibody Name/Abbreviation: CXXC1
CBI Antibody Core Code: A0575

"Lot" Number: 2 Position: 100(63-162)
% Identical Homology of Target to Mouse Protein: 43% (XP_194342.4| RIKEN cDNA 2610014H22)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
SQSRASCPQSTREMSSHRSTTSRRCGVCRGCQCKPCGQCT
YCQDSPQFGGPGVKKQSCIERRCLRVLENRLQRDAPTFKA
RVGCNACEDCRMQDCQICLV


Other western:
Code: A0575
Lot: 1


Western result: +
GST
GST-CXXC1
(fragment)

N.A. kDa (recombinant)
The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.




Sequence Name: CXXC1
Full name:
UniGene Number: N.A.
Accession Number: similar to NM_067178
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 379
NCBI information page: click here

Full length sequence:
MNMMHMGAPMNGGPPPPQMLQMHHPLPHHVAVFAHGSSSQNSQAPPLQAA
SATPPVPSSMNRSQSRASCPQSTREMSSHRSTTSRRCGVCRGCQCKPCGQ
CTYCQDSPQFGGPGVKKQSCIERRCLRVLENRLQRDAPTFKARVGCNACE
DCRMQDCQICLVCLDKRFFENRHIPGAMCAKKRCNNAQSIECVPSLMSQN
PADFVQRAPKRSYELINPVISQQQQQQQSQMHFDVKRVQYMTGPPPQMMQ
QMQMQAPPQQQIPQPQTPQQQVQQHQQRPNSNSSHSQGPSPPRNGVSQQQ
QQQTPPQQQNGVAHQIKTDVNVENLEQVENLEQHQQQQQQQQQQLQQQQQ
RMIFQPAPYPDLSVHARLMVQAPPPPPSL





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© Monday, February 16, 2009 by the CBI Antibody Core and the Center for Biomedical Inventions, University of Texas Southwestern Medical School.