elegant worm (C. elegans) ( Caenorhabditis elegans )
EGL9

Western result: +

GST GST-EGL9 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: EGL9
CBI Antibody Core Code: A0169
"Lot" Number: 1 Position: 100(545-644)
% Identical Homology of Target to Mouse Protein: 56% (NP_444437.1| EGL nine homolog 1; chromosome 1 open reading frame 12; open reading frame 13)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
RNPHEVMPVFRHRFAITIWYMDKSERDKALAKGKESDAAC
ASKKENDPTSSSLNSLIGSLLRPRKNPSTHDLSKLDLRLF
PSTSSDPALVSAADEDRVDI

Sequence Name: EGL9
Full name: EGg Laying defective EGL-9
UniGene Number: N.A.
Accession Number: NM_171533
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 723
NCBI information page: click here

Full length sequence:
MSSAPNDDCEIDKGTPSTASLFTTLMLSQPSSSTAVLQCTYCGSSCTSSQ
LQTCLFCGTVAYCSKEHQQLDWLTHKMICKSLQTSGMVPSNLMPQAAPAV
MAPIPPTVSFDDPALTTSLLLSLQNNPILNQTISNFPPTFSITSKTEPEP
SIPIQIPQRISSTSTVPFSSEGSAFKPYRNTHVFNSISSESMSSMCTSHE
ASLEHMSSASLAMFPTSSTAQSDISRLAQVLSLAGDSPASLALVTTSVPS
TASTATIPPPATTTSSATSSGKSETITVGKEKIIQTDDPDIQIIETEGGS
KPTVSRTRKRPTPSNSADPKINYKDHNKNVVYSTTLQEHQKHLQNRGLAL
SIHQAMVLRLRYIAEHVIRSLNEFGWAVVDNFLGSDHYKFTAKEIERLYE
RGLFSPGQLMEAKHKDEFHIKDIRSDHIYWYDGYDGRAKDAATVRLLISM
IDSVIQHFKKRIDHDIGGRSRAMLAIYPGNGTRYVKHVDNPVKDGRCITT
IYYCNENWDMATDGGTLRLYPETSMTPMDIDPRADRLVFFWSDRRNPHEV
MPVFRHRFAITIWYMDKSERDKALAKGKESDAACASKKENDPTSSSLNSL
IGSLLRPRKNPSTHDLSKLDLRLFPSTSSDPALVSAADEDRVDISADFQS
TSSLAHPESTDSGVSLSTFNVAHNHMERTTSLQSISDHFRSERSHERRSS
TSSDQDLDEGLPPPPSTNPEYYI