elegant worm (C. elegans) ( Caenorhabditis elegans )
rec-8

Western result: +

GST GST-rec-8 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: rec-8
CBI Antibody Core Code: A0744
"Lot" Number: 1 Position: 100(647-746)
% Identical Homology of Target to Mouse Protein: 34% (XP_485939.1| similar to DEP domain containing 1)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
MRTEEELENVRRRQKSSLGVQFMRTDDLEEDTRRNRLFED
EERTRDAREDELFFYSSGSLLPNNRLNIHKELLNEAEARY
PEWVNFNEFTADHDRKKAAT

Sequence Name: rec-8
Full name: Rad21/Rec8 like protein, N-terminal, abnormal RECombination REC-8 (rec-8)
UniGene Number: N.A.
Accession Number: NP_502565
Source organism: elegant worm (C. elegans) ( Caenorhabditis elegans )
Full length size (aa): 781
NCBI information page: click here

Full length sequence:
MVVSAEVIRKDAVFHVAWILGTGDSKKLSRREILDQNLPELCHSIIEMVP
ERHRGSATKTGLYLLSLLTYGTVLIHQVQVDFLKRDVEKLKELMKKKSFI
LLMAERFDRNQELQRKEDKFARLRSKPIMCVEELDRVDLAHLQAIGDELG
INGNPGDFIMMDALPNMNQWIDNNSELNAIYGCVEPYLREKEITMHSTFV
EGNGSNEHNKERRNDAVIADFSQLLFPEIPEITLGEKFPIDVDSRKRSAI
LQEEQEEALQLPKEASEIVQEEPTKFVSIALLPSETVEQPAPQEPIQEPI
QPIIEEPAPQLELPQPELPPQLDAIDLVTIPASQQDMVVEYLQLINDLPD
DENSRLPPLPKDLELFEDVILPPPAKKSKVEEEEDALERARRRPSSRPVT
PINQTDLTDLHSTVRPEDPSFAIDSQIHDVLPQRKKSKRNLPIIHSDDLE
IDEAVQKVLQADYSSLVRKKEDVIAKIPPKTDAVAVLMNLPEPVFSIGYR
LPPEVRDMFKACYNQAVGSPVSDDEEDEDEEEEEEYKYAKVCLLSPNRIV
EDTLLLEEQPRQPEEFPSTDNINPPRQLQENPVFENLEYEAPPHPIRTAR
TPTPIKDLKYSVISLFPTPEKRRETSIIAELNLDPIPVEEIDPLLTMRTE
EELENVRRRQKSSLGVQFMRTDDLEEDTRRNRLFEDEERTRDAREDELFF
YSSGSLLPNNRLNIHKELLNEAEARYPEWVNFNEFTADHDRKKAATAFEG
LLLSLKNMKVEAKQEDPYFPILVRHISHEEM