Streptococcus pneumoniae
SPR1868

Western result: +

GST GST-SPR1868 (fragment)

18.62 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: SPR1868
CBI Antibody Core Code: A1265
"Lot" Number: 1 Position: 42 (486-527)
% Identical Homology of Target to Mouse Protein: 53% (XP_128725.3| NADH dehydrogenase (ubiquinone) flavoprotein 2)
Molecular Weight of fusion protein: 18.62 KDa

Target sequence used to make this antibody:
IATGVNNPENYKDDWDSIVKNLPKGHHMILVTPYEGDKTK
ET

Sequence Name: SPR1868
Full name: Putative acyltransferase
UniGene Number: 605
Accession Number: AAL00670
Source organism: Streptococcus pneumoniae
Full length size (aa): To be posted
NCBI information page:

Full length sequence:
mrikwfsliriiglllvllyhffqtifpggffgvdvfftfsgflitalli
eefsknneidligffrrrfyrivppvvlmvlvtmpftflvrqdyvagigg
qiagvlgfmtnfyelltggsyesqfiphlfvhnwslavevhyyilwglav
wflskqaksngqlkgmvfllsavaflisffsmfigsflvtsyssvyfssl
thvypfflgsmlativgvrqttslvkqldkiwdlrktlvvfgggfgflvl
ltffvkftylfayligfllaslaalamilaarvlhekthhiqeskiisfl
adtsyavylfhwpfyiifsqltsnllavlltlicsygfaslsfyvlepwi
agkntpivqtlrplpyihailaagtgiltiivctvtllapqvgafetdlt
vnglkqaatnigqtkvmaeradanslgiadgtmligdsvalrantalqta
lpgaqinaqvsvttktaneimlnnsqnkflpktvviatgvnnpenykddw
dsivknlpkghhmilvtpyegdktketyaivekaaaymrelaektpyiti
adwnqvakehpeiwagtdqvhfgsesstieagaklyadtiatalqtaqdk
pvksk