Sac. erythraea ( Saccharopolyspora erythraea )
eryA-II

Western result: +

GST GST-DEBS2 A1263a (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: DEBS2
CBI Antibody Core Code: A1263a
"Lot" Number: 1 Position: 100 (2466-2565)
% Identical Homology of Target to Mouse Protein: 42% (NP_032014.1| fatty acid synthase)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
SGRRTIDVHAAEDVADLADAQWSQHATGTLAQGVAAGPRD
TEQWPPEDAVRIPLDDHYDGLAEQGYEYGPSFQALRAAWR
KDDSVYAEVSIAADEEGYAF

Western result: +

GST GST-DEBS2 A1263c (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: DEBS2
CBI Antibody Core Code: A1263c
"Lot" Number: 1 Position: 100 (2846-2945)
% Identical Homology of Target to Mouse Protein: 34% (NP_766604.2| hypothetical protein 9430073I07)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
QPLRAGEVRVDVRATGVNFRDVLLALGMYPQKADMGTEAA
GVVTAVGPDVDAFAPGDRVLGLFQGAFAPIAVTDHRLLAR
VPDGWSDADAAAVPIAYTTA

Antibody Name/Abbreviation: DEBS2
CBI Antibody Core Code: A1263b
"Lot" Number: 1 Position: 100 (2564-2663)
% Identical Homology of Target to Mouse Protein: 32% (NP_065066.1| solute carrier family 12, member 5)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
AFHPVLLDAVAQTLSLGALGEPGGGKLPFAWNTVTLHASG
ATSVRVVATPAGADAMALRVTDPAGHLVATVDSLVVRSTG
EKWEQPEPRGGEGELHALDW

Sequence Name: eryA-II
Full name:
UniGene Number: N.A.
Accession Number: 416966
Source organism: Sac. erythraea ( Saccharopolyspora erythraea )
Full length size (aa): 3567
NCBI information page:

Full length sequence:
MTDSEKVAEYLRRATLDLRAARQRIRELESDPIAIVSMACRLPGGVNTPQ
RLWELLREGGETLSGFPTDRGWDLARLHHPDPDNPGTSYVDKGGFLDDAA
GFDAEFFGVSPREAAAMDPQQRLLLETSWELVENAGIDPHSLRGTATGVF
LGVAKFGYGEDTAAAEDVEGYSVTGVAPAVASGRISYTMGLEGPSISVDT
ACSSSLVALHLAVESLRKGESSMAVVGGAAVMATPGVFVDFSRQRALAAD
GRSKAFGAGADGFGFSEGVTLVLLERLSEARRNGHEVLAVVRGSALNQDG
ASNGLSAPSGPAQRRVIRQALESCGLEPGDVDAVEAHGTGTALGDPIEAN
ALLDTYGRDRDADRPLWLGSVKSNIGHTQAAAGVTGLLKVVLALRNGELP
ATLHVEEPTPHVDWSSGGVALLAGNQPWRRGERTRRARVSAFGISGTNAH
VIVEEAPEREHRETTAHDGRPVPLVVSARTTAALRAQAAQIAELLERPDA
DLAGVGLGLATTRARHEHRAAVVASTREEAVRGLREIAAGAATADAVVEG
VTEVDGRNVVFLFPGQGSQWAGMGAELLSSSPVFAGKIRACDESMAPMQD
WKVSDVLRQAPGAPGLDRVDVVQPVLFAVMVSLAELWRSYGVEPAAVVGH
SQGEIAAAHVAGALTLEDAAKLVVGRSRLMRSLSGEGGMAAVALGEAAVR
ERLRPWQDRLSVAAVNGPRSVVVSGEPGALRAFSEDCAAEGIRVRDIDVD
YASHSPQIERVREELLETTGDIAPRPARVTFHSTVESRSMDGTELDARYW
YRNLRETVRFADAVTRLAESGYDAFIEVSPHPVVVQAVEEAVEEADGAED
AVVVGSLHRDGGDLSAFLRSMATAHVSGVDIRWDVALPGAAPFALPTYPF
QRKRYWLQPAAPAAASDELAYRVSWTPIEKPESGNLDGDWLVVTPLISPE
WTEMLCEAINANGGRALRCEVDTSASRTEMAQAVAQAGTGFRGVLSLLSS
DESACRPGVPAGAVGLLTLVQALGDAGVDAPVWCLTQGAVRTPADDDLAR
PAQTTAHGFAQVAGLELPGRWGGVVDLPESVDDAALRLLVAVLRGGGRAE
DHLAVRDGRLHGRRVVRASLPQSGSRSWTPHGTVLVTGAASPVGDQLVRW
LADRGAERLVLAGACPGDDLLAAVEEAGASAVVCAQDAAALREALGDEPV
TALVHAGTLTNFGSISEVAPEEFAETIAAKTALLAVLDEVLGDRAVEREV
YCSSVAGIWGGAGMAAYAAGSAYLDALAEHHRARGRSCTSVAWTPWALPG
GAVDDGYLRERGLRSLSADRAMRTWERVLAAGPVSVAVADVDWPVLSEGF
AATRPTALFAELAGRGGQAEAEPDSGPTGEPAQRLAGLSPDEQQENLLEL
VANAVAEVLGHESAAEINVRRAFSELGLDSLNAMALRKRLSASTGLRLPA
SLVFDHPTVTALAQHLRARLVGDADQAAVRVVGAADESEPIAIVGIGCRF
PGGIGSPEQLWRVLAEGANLTTGFPADRGWDIGRLYHPDPDNPGTSYVDK
GGFLTDAADFDPGFFGITPREALAMDPQQRLMLETAWEAVERAGIDPDAL
RGTDTGVFVGMNGQSYMQLLAGEAERVDGYQGLGNSASVLSGRIAYTFGW
EGPALTVDTACSSSLVGIHLAMQALRRGECSLALAGGVTVMSDPYTFVDF
STQRGLASDGRCKAFSARADGFALSEGVAALVLEPLSRARANGHQVLAVL
RGSAVNQDGASNGLAAPNGPSQERVIRQALAASGVPAADVDVVEAHGTGT
ELGDPIEAGALIATYGQDRDRPLRLGSVKTNIGHTQAAAGAAGVIKVVLA
MRHGMLPRSLHADELSPHIDWESGAVEVLREEVPWPAGERPRRAGVSSFG
VSGTNAHVIVEEAPAEQEAARTERGPLPFVLSGRSEAVVAAQARALAEHL
RDTPELGLTDAAWTLATGRARFDVRAAVLGDDRAGVCAELDALAEGRPSA
DAVAPVTSAPRKPVLVFPGQGAQWVGMARDLLESSEVFAESMSRCAEALS
PHTDWKLLDVVRGDGGPDPHERVDVLQPVLFSIMVSLAELWRAHGVTPAA
VVGHSQGEIAAAHVAGALSLEAAAKVVALRSQVLRELDDQGGMVSVGASR
DELETVLARWDGRVAVAAVNGPGTSVVAGPTAELDEFFAEAEAREMKPRR
IAVRYASHSPEVARIEDRLAAELGTITAVRGSVPLHSTVTGEVIDTSAMD
ASYWYRNLRRPVLFEQAVRGLVEQGFDTFVEVSPHPVLLMAVEETAEHAG
AEVTCVPTLRREQSGPHEFLRNLLRAHVHGVGADLRPAVAGGRPAELPTY
PFEHQRFWPRPHRPADVSALGVRGAEHPLLLAAVDVPGHGGAVFTGRLST
DEQPWLAEHVVGGRTLVPGSVLVDLALAAGEDVGLPVLEELVLQRPLVLA
GAGALLRMSVGAPDESGRRTIDVHAAEDVADLADAQWSQHATGTLAQGVA
AGPRDTEQWPPEDAVRIPLDDHYDGLAEQGYEYGPSFQALRAAWRKDDSV
YAEVSIAADEEGYAFHPVLLDAVAQTLSLGALGEPGGGKLPFAWNTVTLH
ASGATSVRVVATPAGADAMALRVTDPAGHLVATVDSLVVRSTGEKWEQPE
PRGGEGELHALDWGRLAEPGSTGRVVAADASDLDAVLRSGEPEPDAVLVR
YEPEGDDPRAAARHGVLWAAALVRRWLEQEELPGATLVIATSGAVTVSDD
DSVPEPGAAAMWGVIRCAQAESPDRFVLLDTDAEPGMLPAVPDNPQLALR
GDDVFVPRLSPLAPSALTLPAGTQRLVPGDGAIDSVAFEPAPDVEQPLRA
GEVRVDVRATGVNFRDVLLALGMYPQKADMGTEAAGVVTAVGPDVDAFAP
GDRVLGLFQGAFAPIAVTDHRLLARVPDGWSDADAAAVPIAYTTAHYALH
DLAGLRAGQSVLIHAAAGGVGMAAVALARRAGAEVLATAGPAKHGTLRAL
GLDDEHIASSRETGFARKFRERTGGRGVDVVLNSLTGELLDESADLLAED
GVFVEMGKTDLRDAGDFRGRYAPFDLGEAGDDRLGEILREVVGLLGAGEL
DRLPVSAWELGSAPAALQHMSRGRHVGKLVLTQPAPVDPDGTVLITGGTG
TLGRLLARHLVTEHGVRHLLLVSRRGADAPGSDELRAEIEDLGASAEIAA
CDTADRDALSALLDGLPRPLTGVVHAAGVLADGLVTSIDEPAVEQVLRAK
VDAAWNLHELTANTGLSFFVLFSSAASVLAGPGQGVYAAANESLNALAAL
RRTRGLPAKALGWGLWAQASEMTSGLGDRIARTGVAALPTERALALFDSA
LRRGGEVVFPLSINRSALRRAEFVPEVLRGMVRAKLRAAGQAEAAGPNVV
DRLAGRSESDQVAGLAELVRSHAAAVSGYGSADQLPERKAFKDLGFDSLA
AVELRNRLGTATGVRLPSTLVFDHPTPLAVAEHLRDRLFAASPAVDIGDR
LDELEKALEALSAEDGHDDVGQRLESLLRRWNSRRADAPSTSAISEDASD
DELFSMLDQRFGGGEDL