fruit fly ( Drosophila melanogaster )
Ubp64E

Western result: +

GST GST-Ubp64E A1052a (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Ubp64E
CBI Antibody Core Code: A1052a
"Lot" Number: 1 Position: 100 (475-574)
% Identical Homology of Target to Mouse Protein: 27% (NP_444320.1| down-regulated by Ctnnb1, a)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
GRHITVTDLALPDLYKPRVYFYNPSLKKMKITRVYVSQSF
NINLVLMSAYEMLNVEQFAPLSRCRLVAYNSSMDTIIQSL
ESCTDPALTELRAANYSLDF

Western result: +

control Ubp64E A1052b (fragment)

32.2 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: Ubp64E
CBI Antibody Core Code: A1052b
"Lot" Number: 1 Position: 20 (485-504)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: 32.2 KDa

Target sequence used to make this antibody:
LPDLYKPRVYFYNPSLKKMK

Sequence Name: Ubp64E
Full name: Ubiquitin specific protease 64E
UniGene Number: N.A.
Accession Number: CAA67601
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 898
NCBI information page:

Full length sequence:
MVRTRLCQTSIVASMEAMSCEHGRESTQRVRTSCPRPPQKLRQRQGRRAW
SRGYVGLVNQAMTCYLNSLLQALFMTPEFRNALYRWEFDNDNEAKKSYQL
QKLFLNLQTSPKAAVETTDLTRSFGWDSTEAWQQHDIQELCRVMFDALEH
KFKNTKQANLISNLYEGKMNAYVKCLGCNTEKNDCEDTFLDIPLPVRPFG
SSSAYGSIEEALRAFVQPETLDGNNQYLCEKCKKKCDAHKGLHFKSFPYI
LTLHLKRFDFDYQTMHRIKLNDRVTFPQTLNLNTFINRSGNSGEQNSQLN
GTVDDCSTADSGSAMEDDNLSSGVVTTASSSQHENDLNDEDEGIDMSSST
SKSAKQGSGPYLYELFAIMIHSGSASGGHYYAYIKDFDNNEWFCFNDQNV
TSITQEDIQRSFGGPNGSYYSSAYTSSTNAYMLMYRQVDAKRNELVAKVR
DFPEHIKTLLPKLHSEEETRVSRLGRHITVTDLALPDLYKPRVYFYNPSL
KKMKITRVYVSQSFNINLVLMSAYEMLNVEQFAPLSRCRLVAYNSSMDTI
IQSLESCTDPALTELRAANYSLDFLLEYRAEDQEFEVYRERHYLVCVKVD
LSTMAMDGPLVTQQGERKPHVLRAIALRCTLASSSSYSHGARHGAKAFVS
YDPHPTRRPCNISRTWPTPQFKSITYFYLNVPNTDAATLEMLGVPTVESV
ECASGGDVVDAAMMNGVAPGHMSSSNDYDWRRYKRDLVEPMSQPSPSHGH
ESNSEDSSLSDGDRTLVETDNMAHRGGGDSQVSSTSHSPQLSSPEDEAAS
HDAMMRVHAYCNGNGSYAAADVVDPLLLPTSTNHFFYRHKGRVRGCGWHR
FQSGHQSDEEAQLRTRTRAYKLLVGTHMRMGAFKKHHRAADPSARRPL