fruit fly ( Drosophila melanogaster )
Gr10a

Western result: +

control Gr10a A1105b (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag). The negative control lane is the same except it contains ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used.

Antibody Name/Abbreviation: Gr10a
CBI Antibody Core Code: A1105b
"Lot" Number: 1 Position: 20 (1-20)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of GST-fusion: N.A. KDa

Target sequence used to make this antibody:
MTSPDERKSFWERHEFKFYR

Antibody Name/Abbreviation: Gr10a
CBI Antibody Core Code: A1105a
"Lot" Number: 1 Position: 100 (100-199)
% Identical Homology of Target to Mouse Protein: 45% (NP_683748.1| solute carrier family 8 (sodium/calcium exchanger), member 2)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
ATVIVTYVANTVHFEAINQRCTMQRTHLEFEFKNAPQEPK
RPFEFFMYFKFCLINLMMMIQVCGIFAQYGEVGKGSVSQV
RVHFAIYAFVLWNYTENMAD

Sequence Name: Gr10a
Full name: CG32664-PA
UniGene Number: N.A.
Accession Number: P58950
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 408
NCBI information page:

Full length sequence:
MTSPDERKSFWERHEFKFYRYGHVYALIYGQVVIDYVPQRALKRGVKVLL
IAYGHLFSMLLIVVLPGYFCYHFRTLTDTLDRRLQLLFYVSFTNTAIKYA
TVIVTYVANTVHFEAINQRCTMQRTHLEFEFKNAPQEPKRPFEFFMYFKF
CLINLMMMIQVCGIFAQYGEVGKGSVSQVRVHFAIYAFVLWNYTENMADY
CYFINGSVLKYYRQFNLQLGSLRDEMDGLRPGGMLLHHCCELSDRLEELR
RRCREIHDLQRESFRMHQFQLIGLMLSTLINNLTNFYTLFHMLAKQSLEE
VSYPVVVGSVYATGFYIDTYIVALINEHIKLELEAVALTMRRFAEPREMD
ERLTREIEHLSLELLNYQPPMLCGLLHLDRRLVYLIAVTAFSYFITLVQF
DLYLRKKS