fruit fly ( Drosophila melanogaster )
klu

Western result: +

GST GST-klu A1107 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: klu
CBI Antibody Core Code: A1107
"Lot" Number: 1 Position: 100 (192-291)
% Identical Homology of Target to Mouse Protein: 26% (NP_061293.1| nuclear factor of activated T-cells 5 isoform b; tonicity-responsive enhancer binding protein; Rel domain-containing transcription factor)
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
EQDEDHDGSVPDEEDDSVDQDGDAGKYIKSEQPVSEDEEN
EQEMDQDIEDISGEDLELSHSDDNDQDDSPRISMSPLQLQ
AAQSNLPQDQNQPMNKENPL

Antibody Name/Abbreviation: klu-N
CBI Antibody Core Code: A1109
"Lot" Number: 1 Position: 100 (11-109)
% Identical Homology of Target to Mouse Protein: 37% (XP_110931.4| PREDICTED: RIKEN cDNA 1110001M19)
Molecular Weight of fusion protein: 24.89 KDa

Target sequence used to make this antibody:
GHGHHTNPHNPPQQPPQHHPHHLPLPHPPPAPPPQLLNFN
HHQQQYLPHMYPAAAAAAQSHSHYGGLQYPHPHALQHQHH
PHQHQHHLPLALSLHQQAA

Antibody Name/Abbreviation: klu2-4
CBI Antibody Core Code: A1108
"Lot" Number: 2 Position: 87 (623-709)
% Identical Homology of Target to Mouse Protein: 53% (NP_035893.1| zinc finger protein 9)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
AKAYSCDVCRRSFARSDMLTRHMRLHTGVKPYTCKVCGQV
FSRSDHLSTHQRTHTGEKPYKCPQCPYAACRRDMITRHMR
THTRYDS

Sequence Name: klu
Full name: klumpfuss
UniGene Number: N.A.
Accession Number: Y11066.1
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 803
NCBI information page:

Sequence Notes: klu-N = N-terminus

Full length sequence:
MTMAEGTDPMGHGHHTNPHNPPQQPPQHHPHHLPLPHPPPAPPPQLLNFN
HHQQQYLPHMYPAAAAAAQSHSHYGGLQYPHPHALQHQHHPHQHQHHLPL
ALSLHQQAAAAAAVAAAVATNHSNNNNQASVANNNIVVPWKQRKRKRLSA
VLDKLHHNNNNNNNNNNETHSNGMACKAEPMEMKGDLAEDAEQDEDHDGS
VPDEEDDSVDQDGDAGKYIKSEQPVSEDEENEQEMDQDIEDISGEDLELS
HSDDNDQDDSPRISMSPLQLQAAQSNLPQDQNQPMNKENPLHVDIKTEIP
SPYDRYFPIPSPLFGYYLHTKYLNEVFRRRHDLYPSPLQHTPSSIASETE
TSPNAQERKSSSNHVLPHALLANNSPPPSLPSPPRSESSVTNNVATTTTS
STTKKRSSPKPKGKKGEKKPMPPPQERPLDLCMRNEVEPKKYKKSGSKSS
LESRSAGMMPPPPALSAASSLESMSALSPASSSHSGHMPITSAATPNHQP
PPNSPYANAMNAAHAAAAAAAAAMIKMEMPLHPLHHQQMHHSQVPTTTVG
VPVIKGDVASPTTKETVAWRYNLDVSPVVEEMPPGSDVAYVCPTCGQMFS
LHDRLAKHMASRHKSRNPANDIAKAYSCDVCRRSFARSDMLTRHMRLHTG
VKPYTCKVCGQVFSRSDHLSTHQRTHTGEKPYKCPQCPYAACRRDMITRH
MRTHTRYDSRGGSREGREGREGREGKESRDSRRSNERIEEPNSPGSHSLL
DMKMNMNMGMGMGLNMGLPMNPMSLLQEELLQKSQPGLTLGGPMPLVVKT
ESA