fruit fly ( Drosophila melanogaster )
CG9311

Western result: +

GST GST-CG9311 (fragment)

25 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: CG9311
CBI Antibody Core Code: A1244
"Lot" Number: 1 Position: 100 (1004-1103)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 25 KDa

Target sequence used to make this antibody:
QQLTPQQQTGYSQSQAPQLQAPSYAQNQQSTGQAQQQQTG
YPQTPQQHLAGYAQSQQQATGYPQSQTSQQQTTGYSQSQT
PQQPLAYQQQPSGYPQQQQQ

Sequence Name: CG9311
Full name: Drosophila melanogaster SD03094 full length cDNA
UniGene Number: Dm.4388
Accession Number: AY052076
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1834
NCBI information page: click here

Full length sequence:
MEAVPRLHMLWFALKSSPEGTSFAALKKYIAEFYHEDPEAYSKEVHALET
LRNQAMHTTKDGAPVMKRYYCQLHALQNRFPQLADRGIFTFKWKDLYHSA
VHEVTDLRFERAAVLFNIAALHTQSGASVTRGDVDGMKMACTHFQAAAWA
YGELRERYANVNGGGDFMTPELLVFQQQVCFAQAQECILEKSLIDNRKPH
IVAKVTAQIVVYYGAALAALLTGGDDGPVAQVIDSSVYKLWKKYVRFKIN
YLTCILYLYQGQHSEEKRQMGERVTLYQASWDKLEEARKESKGLPDQREI
NESLSFTADVVEAKRKNAKNENEFIYHEAVPELSTIAAVQGANLVNGIGF
QVSDEEHAGPDIFARLVPMKAHEASSLYSEEKAKLLRKYGALLEEKDTQL
ESYMSSLTLDNLNINEEQANKLPQGIVDRCAALNANKTAISDLVEAMSQL
AEITADVETNLGEISSMLEAEAKAEREFQSATGVQRTPNAHITELSREFQ
KYSEAHARAGESNNTLRKAMSLHVNNLKILARPLPEIQQLMPKLSSELNT
TEIFRDVKLILNKVNEMKAQRAQFHADLRIAINEDDITGKVIAHGGQEGL
QALFATELGKHDKITELLDQNMVAQGNILQALTENYAKAAPVLKTLQDVK
QKREHFYSSLAASYDVYEDLLAKSAKGLEFYKKLAGNVQKLLTRFRSARD
VQSEERQQRMQSVKAATTPVVSKPIAETTPVPAVSSTPKLRDYLKAKAAM
ADASNPAYVPTVRPVPVGSENPTQAACSYATAPPNEPPPPYSLTQQQYFD
PSAAGYTNPMYQQQQQHIAPPAYKAQASPNSQTLHGALGQMNLQGQSQDN
NQAGMAYNYGYPSGSVPPLAYAPPGTVPASSASQGLNIQQPLYVATSGNP
VSVSNPSELPSTLQAPFVVNPMSNPYQTAAGYSGQAYQQVNAQAQQVTYP
TAPSGSSTGQSAQQPLGYAPSQGPQQQAGGYPQSQTPQQQVGAYQQQPLS
GYAQQLTPQQQTGYSQSQAPQLQAPSYAQNQQSTGQAQQQQTGYPQTPQQ
HLAGYAQSQQQATGYPQSQTSQQQTTGYSQSQTPQQPLAYQQQPSGYPQQ
QQQQSLAPQQPSLYPSQAVQASPQPIPGQQATPPNAGQPLPYPQQYTDPN
AAPAPAPGVSSVPSPVPVPTPAPTSSQAQVPTSPHPSATYTSYSNHPGYS
FNPQTGKYEYSSGYQQDTSSLKGSQASQSYQFSQSGKQQSVGTTDSEIAS
RSNTATGFDSPIVSHTKVNMAAKDSTSAIEGNESSNYYSTPYGYLGSVNP
QQQPTPAPPSNSTASIYMQSGASSTAETQTTSSGVPYASSAALTKVEPKP
EALPPKVTSNVDLLSDLDIDCSVAVPPPMLPQPVLQPQVVATPTPPASQV
SVPVKVESVAEHAAPATAEIPVAATEGTAQTPVTIPSGPKCASLDNLSNC
SDLSSLENFDWDSVSVTHSVSEKQTKASATANGHSSSFAFQSETFTDEKT
TKYFQKEVESYEKLLENLHVKMLNGKTQLGAKWQELQQKLDKEAAANKRS
TTIAKLFPEKNRSLDCLPYDHARVKLDKQTDDYINAAYMKNLSAGCPNFI
VAQTPQPNTINDFWSMIWSEKSRTVVCLHTPNELFDPYWPQALDQPTHYD
DYTVTCLKVQQLSHCSEYQLKLSMHGADAVLDLSLLQLKQWTKGAPAQLL
GVAENALETHRQRCKAANAPQSPLIMNcLTGSErSELVAIGVCAIIATQN
KQPILLNTIDVWSRICAQRQNSLRDSAILEQSMQIVLCNAHNVLNKRGIM
TSYQMKMAPQVTAKEQQENKDPLNELDALWKLK