fruit fly ( Drosophila melanogaster )
BAP155

Western result: +

GST GST-BAP155 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: BAP155
CBI Antibody Core Code: A0714
"Lot" Number: 2 Position: 100(1105-1204)
% Identical Homology of Target to Mouse Protein: 32% (NP_848140.2| YLP motif containing 1; nuclear protein ZAP; ZAP3 protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
PTAQHQPLPPHVVSSPNGAPFAAPPISLTGGLPPGAPTAI
VTNPSDQTGPVAAGAAQSQAPTPMDTTPPSSGPVSDANAP
PGSAMPPGGIPPANTAPIAG

Sequence Name: BAP155
Full name:
UniGene Number: N.A.
Accession Number: AAC28454
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1209
NCBI information page: click here

Full length sequence:
MNTLGPKKDGSPNIDFFQSPETLQGFESIRQWLQKNCKKYLAHSSEPITK
ESLAQLLIHFLQYVEAKLGKNSADPPATRIPMRCFLDFKSGGGLCIIFST
MFRFRAEQRGKKFDFSIGKNPTRKDPNIQLLIEIEQALVEADLYRIPYIY
IRPEIEKGFEGKLREILDNRRVEIVSDEEDATHIVYPVVDPHPDEYARPI
FKRGGHVMLHWYYFPESYDSWAVNNFDLPDHIPENPESPAERWRVSASWI
VDLEQYNEWMAEEDYEVDEQGKKKTHKQRMSIDDIMSFGDEKKKPAASSG
GGKQKRRRSPSPASSASTSKPGKRKRSPAVVHKKSRNDDDDEDLTRDLDD
PPAEPNVQEVHKANAALQSTASPAPGGKSRGDNDMMPIKGGTMTDLDDEM
TGGSAAQAMSTGDGENSQTGKTSDNSNTQEFSSSAKEDMEDNVTEQTHHI
IVPSYSAWFDYNSIHVIEKRAMPEFFNSKNKSKTPEIYMAYRNFMIDTYR
LNPTEYLTSTACRRNLAGDVCAIMRVHAFLEQWGLINYQIDADVRPTPMG
PPPTSHFHILSDTPSGLQSINPQKTQQPSAAKTLLDLDKKPLGKDGGLEL
GDKSGLTGIKTEALENGAAGGLSSGVSQFGLKLDQYAKKPAAMRNRTAAS
MAREWTDQETLLLLEGLEMHKDDWNKVCEHVGSRTQDECILHFLRLPIED
PYLEDDGGFLGPLACQPIPFSKSGNPIMSTVAFLASVVDPRVAAAAAKAA
MEEFAAIKDEVPATIMDNHLKNVEKASAGGKFNPNFGLANSGIAGTGNDK
DDEEGKEGGASASAGGSDEEMKDLSKKDDDAKSKDNTKSDKTNTNTDSSS
TSSSATGNTNNTDKKPKESSGSSPSGDKSAIKSDKSNKSSPTETAAAASG
GEVDIKTEDSSGDGETKDGTEAKEGTGTGTGPLAVPKEGTFSENNMQTAA
AAALASAAVKAKHLAALEERKIKSLVALLVETQMKKLEIKLRHFEELEAT
MEREREGLEYQRQQLITERQQFHLEQLKAAEFRARQQAHHRLQQELQGQA
AAGSMILPQQQQLPQAQQPQQQQQTLPPHPHLAQQQQLPPHPHQLPPQSQ
PLAGPTAQHQPLPPHVVSSPNGAPFAAPPISLTGGLPPGAPTAIVTNPSD
QTGPVAAGAAQSQAPTPMDTTPPSSGPVSDANAPPGSAMPPGGIPPANTA
PIAGVAPSS