fruit fly ( Drosophila melanogaster )
cycle (BMALI)

Western result: +

GST GST-cycle (BMALI) (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: cycle (BMALI)
CBI Antibody Core Code: A0738
"Lot" Number: 2 Position: 100(179-278)
% Identical Homology of Target to Mouse Protein: 42% (NP_031515.1| aryl hydrocarbon receptor nuclear translocator-like)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
LIDAKTMLPVKTDVPQSLCRLCPGARRSFFCRMKLRTASN
NQIKEESDTSSSSRSSTKRKSRLSTGHKYRVIQCTGYLKS
WTPIKDEDQDADSDEQTTNL

Sequence Name: cycle (BMALI)
Full name: bHLH-PAS protein CYCLE (cycle)
UniGene Number: N.A.
Accession Number: AF065473
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 413
NCBI information page: click here

Full length sequence:
MEVQEFCENMEEIEDENYDEEKSARTSDENRKQNHSEIEKRRRDKMNTYI
NELSSMIPMCFAMQRKLDKLTVLRMAVQHLRGIRGSGSLHPFNGSDYRPS
FLSDQELKMIILQASEGFLFVVGCDRGRILYVSDSVSSVLNSTQADLLGQ
SWFDVLHPKDIGKVKEQLSSLEQCPRERLIDAKTMLPVKTDVPQSLCRLC
PGARRSFFCRMKLRTASNNQIKEESDTSSSSRSSTKRKSRLSTGHKYRVI
QCTGYLKSWTPIKDEDQDADSDEQTTNLSCLVAIGRIPPNVRNSTVPASL
DNHPNIRYVLFISRHSGEGKFLFIDQRATLVIGFLPQEILGTSFYEYFHN
EDIAALMESHKVVMQVPEKVTTQVYRFRCKDNSYIQLQSEWRAFKNPWTS
EIDYIIAKNSVFL