fruit fly ( Drosophila melanogaster )
kohtalo

Western result: +

GST GST-kohtalo (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: kohtalo
CBI Antibody Core Code: A0626
"Lot" Number: 1 Position: 100(2077-2176)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
APQQQQPPQPYHNQYAPHQQNMQSNRIERPPLNANSKQAL
SQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQQPGPQQ
QMNPNQMRQQQMNPQQNPQS

Sequence Name: kohtalo
Full name: kohtalo CG8491-PA
UniGene Number: N.A.
Accession Number: NP_524786
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 2531
NCBI information page: click here

Full length sequence:
MLSMLQEKRPLKRTRLGPPDIYPQDAKQREDELTPTNVKHGFTTTPPLSD
EFGTAHNSNVNASKVSAFFSGVLAKKEELMTLPDTGRKKQQINCKDNFWP
VSPRRKCTVDAWFKDLAGNKPLLSLAKRAPSFNKKEEIFITLCENQVNMQ
RATWFIKLSAAYTLSFTESKNKKRSIYDPAAEWTGNMIKFMKELLPKLQE
YYQQNHDKSSSNGTTSGSLTAAGNGPASNGSTGTSSINSVTGSSASTNVI
PVPSMASPLPPIHSPANGQQAAPGGGVNAGSVMPTTGSLGGVVGGPGSSV
VGGAAGAGAAVPPGSTISGIGSQFEDSRNALKYWKYCHQLSKYMYEESLL
DRQEFLNWILDLLDKMRTQASFDEPLKKLVLSFALQYMHDFVQSERLCRK
MAYIVSKKLAQLLNTVVEQQTIKELDEPKLQQDPYELALQEQMSCPHHRD
IVLYLSTILQIITIECPTALVWSGIAAHRAPSSLLGSPLDHLPLAPSVLP
MPTRCPRTNHEIRRQLRAAESDIVLRTQHAEQRWFAAKWLSAGKNQYTSV
LATLDHLDTHCFDRMEHNNSIDTLYAQIFPSPTVSRRREEDQVEPRPPYE
PKQDKDTVRILCEWAVSGQRWGEHRAMVVAILLDKRQIDVTSTPADQQSS
DKDDKDSLASGAGLIDGLPVFQHVLMHFLDHDAPVLDEHVSSPQQRTEFT
NLVQLFSALIRHDVFSHNAYMHTLISRGDLLLESVLVIKSGTTATKTSPP
PPAPPPTTTHGFDDDGFGGGLDFKHNEFDDSNVDDDLDKLVQNIKEKGQQ
HEAPDSPKIGPPGDGETNPGGSISRHYVYTKHFPIPQDDPSMSSYSSESN
QRYILLFGVGKERDEKKHAVKKMSKEIGKLFTKKFSIDVAAAGHVKKHSR
NEFNFEATTSKCQQMAYFDQHVVTAQCAANVLEQLNGFALGNNNYLPVQE
HVAFLFDLMELALNIYSLLELCDSLLKELPEVEHQLQLKKSNLVRSYTTS
LALYIVSILRRYHSCLLLSPEQTLSVFEGVCRTIRHVSNPSECTSAERCI
IAYLSDLHESCVLLQGKEQSTEYYQQLQCIKRFKDIFNTPEQLDLPPQGY
NPLLLQELFMAPRRGGKLDPHWLGTLHESPANVYSFVSNALIAVCRETDN
ERLNDVALACAELTASCNVLSEEWIYALQSLCSGSKSPRYPHLGGQVDIG
QLKTHNALAVFVCILVARHCFSLADFVSKFALPTLARSVSAGGAELSVDA
EAGARLTCHLVLKLFKTLEIPQPGMYSVSTSPNPLHAVGNDFSIRLSCDR
HLLVGAHKTIPIAAVLAVLKAILIVVDNAALKTPLASGSGTSSGGLGGAF
GSGKRSGFNTPVHPGSTPKSNEQRPADLSQILGTSDLQLGSSLTSEPEAL
QQPSVGGMEQISLLEFAQAVLKQICAQEHVLERCLKNAEQLCDMIIDEML
TAKQAQRVLHMICYPEPEFNIISELDQRSMIVRILENLGQWTLRISWLDL
QLMYRQSLSNNAELNVWLDTVARAAIDVFHMEEVVLPGAVKATHKPKPST
WLVAPLIAKLTPAVQGRILRVAGQVLESMNYFSKVSKSDCNSSGSGDERE
KSNSCHSSNSYGLGGVPARNKKMPLDYQPFLGLILTCLKGQDEYKENLLV
SLYAQLSQCLQSFAELDTIGGIDEPQAREEILDALQLRFSLVGGMFEAIQ
KNSTPTTDWAILLAQLVCQGVVDLSCNRELFTTVVDMLATLVHSTLVSDD
ERHYMNLMKKLKKEIGEKNNASIRVIRQLLPLYKQPTEVIACEHSGMDTK
GNKICDIDKKQLRISDKQRISVWDILEGHKNPAPLSWVWFGAVKLERKPL
TYEEAHRNLKYHTHSLVKPSSYYYEPLPLPPEDIEPVPEKICIKDEMKAD
TPSSVDQSPSAVVGGTGRGRGKGTTTRKRKPKNPKTPPVVNTQQQQPQLA
QQPQQPQNVQQQQLQQQQQQQQHMQQQHMQQQQMQPNQMGQMPMNMPMNM
QQFAPNPNNMMQQNAMLQQQQQQQMQQMGNNPMQQQLNVGGGNGQPNPQM
NFMQQGPGGGGAGPQGMPGQQQQWHNAPQQQQPPQPYHNQYAPHQQNMQS
NRIERPPLNANSKQALSQMLRQRQPFQQQAQQGPGGGFNPMQQQPQASQQ
QPGPQQQMNPNQMRQQQMNPQQNPQSVAAFNAMQQQPQQNAQQQQMNPNQ
QQQQQFMRGGNMRPGMAPNQMNQMNMGGQGMSQNPMMQQQIPQNMVGMVN
PNANQMMQSGGAQGGNGVGVGVGVGVGGAGNNPNMGMGGMPQQGMIQQQP
QQQPQQQVQFQNFQNQYQQQQQQGMQQQGGGAGVGVGVGMAPNQQQQQQA
NMMGNFNPQMQQGNRNNPDFMAAAAVAQQQQQQQQQQRVVPGGMMAGNRN
QYMNQAPNVTMSTMMGPGPGGVVGQVPPYARQQSAGGGKPGVLNTQQQFQ
QQQQQQQQLRHQMMQLQGMGGGAGGGMGAGPQQGGGAVGGGAGGGMVPQQ
QSMNQQQTPNLVAQLQRQNMMGQQQYQPPPY