fruit fly ( Drosophila melanogaster )
PARP

Western result: +

GST GST-PARP (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: PARP
CBI Antibody Core Code: A0684
"Lot" Number: 1 Position: 100(541-640)
% Identical Homology of Target to Mouse Protein: 50% (NP_031441.2| ADP-ribosyltransferase (NAD+, poly (ADP-ribose) polymerase) 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 1)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
GLTDIQRNKNSYYKVQLLKADKKEKYWIFRSWGRIGTNIG
NSKLEEFDTSESAKRNFKEIYADKTGNEYEQRDNFVKRTG
RMYPIEIQYDDDQKLVKHES

Sequence Name: PARP
Full name: poly (ADP-ribose) polymerase
UniGene Number: N.A.
Accession Number: NP_524710
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 994
NCBI information page: click here

Full length sequence:
MDIELPYLAEYARTGRATCKGCKSTISKDTLRIAVMVQSAFHDAKVPNWF
HKTCFFKNQRPSSVGDIQNIGNLRFADQKELTDLVENIQEVISAQLGKKR
SKAFNLALKDFGIEYAKSSRSTCRGCEQKINKDLVRLRKTVYDTEVGMKY
GGQPLWHHLECFAQLRSELGWFASGEDMPGFQSLADDDQAKVKNAIPPIK
SEELPDTKRAKMELSDTNEEGEKKQRLKDQNDAYFRFRDDIKNKMKKKDI
DILLKFNNQQPVTGDTEKLFDQTADLLTFGAIESCSECNSCQFIVNKSGY
ICNGNHSEWTKCNKLLKEPTRSACIVPKELKALYNFLNTVKEIPSTRIFN
NFPPNKSTFSRSLLKTNKNNDVLVRPTIPRISPPLYNLKFSIIGLKNQHK
ELRKRIENLGGKFEVKISENTIAIISTELEIQKKSTRMKFAEELGIHIVP
IEFLDFVEADTEGAIKYINSTCICSWGTDPKSRIPKETTKSLNSNSIYTK
SMPVSRTFKVKDGLAVDPDSGLEDIAHVYVDSNNKYSVVLGLTDIQRNKN
SYYKVQLLKADKKEKYWIFRSWGRIGTNIGNSKLEEFDTSESAKRNFKEI
YADKTGNEYEQRDNFVKRTGRMYPIEIQYDDDQKLVKHESHFFTSKLEIS
VQNLIKLIFDIDSMNKTLMEFHIDMDKMPLGKLSAHQIQSAYRVVKEIYN
VLECGSNTAKLIDATNRFYTLIPHNFGVQLPTLIETHQQIEDLRQMLDSL
AEIEVAYSIIKSEDVSDACNPLDNHYAQIKTQLVALDKNSEEFSILSQYV
KNTHASTHKSYDLKIVDVFKVSRQGEARRFKPFKKLHNRKLLWHGSRLTN
FVGILSHGLRIAPPEAPPTGYMFGKGIYFADMVSKSANYCCTSQQNSTGL
MLLSEVALGDMMECTSAKYINKLSNNKHSCFGRGRTMPDPTKSYIRSDGV
EIPYGETITDEHLKSSLLYNEYIVYDVAQVNIQYLFRMEFKYSY